We narrowed to 4,520 results for: gca

1. pCG-ACTB-C4

   Plasmid

   #229846

   Purpose

   Expresses wild-type Cas9 and gRNA for ACTB gene.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDual_dsCas9_Venus_hs_NQO1

   Plasmid

   #214684

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1

   Depositor

   Kevin Janes

   Insert

   dgRNA_NQO1 (NQO1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCP6

   Plasmid

   #166108

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNSU299_YDR344C

   Plasmid

   #166072

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-gRNA-BTRC-MGMT_2

   Plasmid

   #136416

   Purpose

   Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

   Depositor

   Massimo Squatrito

   Insert

   U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE4F1.1.0-gDNA

   Plasmid

   #132451

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   E4F1 (E4F1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF700.1.0-gDNA

   Plasmid

   #112481

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF700

   Depositor

   Kevin White

   Insert

   ZNF700 (ZNF700 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRbeta shRNA #1

   Plasmid

   #32620

   Depositor

   Connie Cepko

   Article

   Billings et al PLoS One. 2010 . 5(10):e13739.

   Insert

   TRbeta shRNA #1 (THRB Chicken)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pBW004

    Plasmid

    #131805

    Purpose

    Vector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    Zea mays FNR and Z. mays SIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. shGFP neo

    Plasmid

    #72571

    Purpose

    Hairpin targeting GFP.

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. LT3GEPIR-CDK12shRNA

    Plasmid

    #220583

    Purpose

    To inducibly knockdown CDK12 expression

    Depositor

    Sean Lee

    Insert

    CDK12 shRNA (CDK12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOttc438 - pAAV EF1a CRE ERT2 bGHpA

    Plasmid

    #210415

    Purpose

    AAV viral vector packaging plasmid that expresses tamoxifen-regulated Cre recombinase

    Depositor

    Christopher Richie, Brandon Harvey

    Insert

    Cre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUPER-DsRed

    Plasmid

    #42053

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    DsRed shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pELF2.1.0-gDNA

    Plasmid

    #132454

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ELF2 (ELF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-PPP2R5D-2

    Plasmid

    #179371

    Purpose

    shRNA suppression

    Depositor

    William Hahn

    Article

    Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

    Insert

    B56δ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Age1

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZBTB9.1.0-gDNA

    Plasmid

    #112469

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9

    Depositor

    Kevin White

    Insert

    ZBTB9 (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZBTB17.1.0-gDNA

    Plasmid

    #112483

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17

    Depositor

    Kevin White

    Insert

    ZBTB17 (ZBTB17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPDEF.1.0-gDNA

    Plasmid

    #113796

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF

    Depositor

    Kevin White

    Insert

    SPDEF (SPDEF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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