-
Plasmid#117050PurposeIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoterDepositorInsertaadA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHInt-AppA-KanR-loxP
Plasmid#232910PurposePlasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.DepositorInsertAppA (appA )
UseSynthetic BiologyTagsExpressionMutationPromoterPj5Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-Tev-MS2-p65-HSF1
Plasmid#84505PurposeMESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1DepositorInsertsV1-MESA-45F-Tev
MS2-P65-HSF1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-neoR
Plasmid#117048PurposeIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoterDepositorInsertneoR
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22C
Plasmid#91135PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23C
Plasmid#91140PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS148∙CsR
Plasmid#197858PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Ap/Cb resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only