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Showing: 4751 - 4800 of 6939 results

  3. pOPO794

    Plasmid
    #195303
    Purpose
    pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade Δcas6 and ribozyme flanked entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195303-plasmid-map-sequence-id-383144

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  7. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  8. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

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  13. pCAG-mSpata33 (I81A)-PA

    Plasmid
    #179131
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 81 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179131-plasmid-map-sequence-id-352818

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  15. pCAG-mSpata33 (P79A)-PA

    Plasmid
    #179130
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Proline 79 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179130-plasmid-map-sequence-id-352804

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  16. pCAG-mSpata33 (I83A)-PA

    Plasmid
    #179132
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 83 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179132-plasmid-map-sequence-id-352797

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  21. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  23. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

    Plasmid
    #168491
    Purpose
    Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168491-plasmid-map-sequence-id-332602

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  31. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Use
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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  48. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    YFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

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Showing: 4751 - 4800 of 6939 results