We narrowed to 4,298 results for: ER;

1. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209016

   Purpose

   Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209011

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX SYT9 C2A D197,199N

   Plasmid

   #195704

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 124-211 of mouse SYT9 with D1…

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX SYT9 C2B D330, 332N

   Plasmid

   #195705

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 253-344 of mouse SYT9 with D3…

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC-cUnaG

   Plasmid

   #207631

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC-LPETG

   Plasmid

   #207629

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SiRES COR1C ACT-DCC-Halo

   Plasmid

   #195148

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SiRES COR1C ACT-DCC-GFP

   Plasmid

   #195147

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-RedO-Txnip(C247S)

   Plasmid

   #206341

   Purpose

   AAV plasmid expressing mutant TXNIP in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   C247S

   Promoter

   human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFsynW SYT1-PGM reporter

    Plasmid

    #197278

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI pHluorin Syt12

    Plasmid

    #184513

    Purpose

    Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 12 (Syt12 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI pHluorin Syt17

    Plasmid

    #184514

    Purpose

    Mammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 17 (Syt17 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI pHluorin Syt9

    Plasmid

    #184510

    Purpose

    Mammalian expression of Synaptotagmin 9 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 9 (Syt9 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI pHluorin Syt8

    Plasmid

    #184509

    Purpose

    Mammalian expression of Synaptotagmin 8 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 8 (Syt8 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI pHluorin Syt3

    Plasmid

    #184504

    Purpose

    Mammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 3 (Syt3 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30_Halotag_K73T_Jdomain

    Plasmid

    #183693

    Purpose

    Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)-J domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCh-Miro1(∆TM)-SspB

    Plasmid

    #194007

    Purpose

    Optogenetic tool

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dora[B]_rescue_construct

    Plasmid

    #190637

    Purpose

    Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLS.407

    Plasmid

    #187204

    Purpose

    Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v32
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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