We narrowed to 10,177 results for: HAE

1. TFORF1850

   Plasmid

   #142870

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAE (YWHAE Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mutant Human Cortactin-GFP

   Plasmid

   #71365

   Purpose

   mammalian expression of mutant human Cortactin-GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   cortactin (CTTN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutation present at 72nd nucleotide

   Promoter

   CMV

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET30a-SARS-CoV2-ORF3b (S23Q)

   Plasmid

   #165088

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Stop codon to glutamine at 23rd amino acid

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAS_4xG1 PylT FLAG-G1 PylRS

   Plasmid

   #154768

   Purpose

   plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-KCC2

   Plasmid

   #104075

   Purpose

   expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

   Depositor

   Igor Medyna

   Article

   Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   CMV

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-Spike-403-505

   Plasmid

   #165095

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pBABEpuro-Flag-YAP2

   Plasmid

   #27472

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

   Plasmid

   #127790

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. shGLDC-DOX

   Plasmid

   #72883

   Purpose

   dox inducible knockdown

   Depositor

   David Sabatini

   Article

   Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

   Insert

   RNAi hairpin against GLDC
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-SwitchON-sgCh2-2

    Plasmid

    #199637

    Purpose

    Tamoxifen-inducible expression of sgRNA control targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-Fbxo7(ΔF-box)-IRES-GFP

    Plasmid

    #171787

    Purpose

    retrovirus production

    Depositor

    Heike Laman

    Article

    Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

    Insert

    Fbxo7 isoform 1 (FBXO7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    deletion of aa335-367

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2Flag CMV2-ZO-2-N

    Plasmid

    #27418

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of the carboxy-terminus of ZO-2, so only…

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pMSCV_PIG_3XHA-Ago1

    Plasmid

    #170916

    Purpose

    Expresses 3X-HA-AGO1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    Ago1 (Ago1 Mouse)
    

    Use

    Tags

    3X-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS663-pMCSG7-AcrIIC4Hpa

    Plasmid

    #113440

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124545

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124544

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124540

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge