We narrowed to 165,879 results for: addgene

1. VV063: 1xCox8 - superecliptic pHluorin in fck

   Plasmid

   #58500

   Purpose

   Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - Superecliptic pHluorin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. JAK3 (JAK3A-c023)

   Plasmid

   #98229

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JAK3A (JAK3 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Mutation

   pfam00069:Pkinase 822-1091

   Promoter

   polyhedrin

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6CCR (GID4)

   Plasmid

   #119960

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 116-300

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. VV064: 1xCox8 - ratiometric pHluorin in fck

   Plasmid

   #58502

   Purpose

   Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - Ratiometric pHluorin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3814

   Plasmid

   #154122

   Purpose

   mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p::rtetR-L-QFAD::act-4 '3
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5T5G (SETD8)

   Plasmid

   #101886

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD8 (KMT5A Human)
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-BC-start

   Plasmid

   #127168

   Purpose

   Entry plasmid for two-step guide-barcode cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGlnA-Ypet

   Plasmid

   #98278

   Purpose

   mammalian expression of GlnA-Ypet

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   GlnA
   

   Tags

   Ypet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNM3724

    Plasmid

    #154112

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p6VP1

    Plasmid

    #24725

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 6
    

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSADdeltaG-GFP-mCherry

    Plasmid

    #32643

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    mCherry
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pFtnA-Halo

    Plasmid

    #98281

    Purpose

    mammalian expression of FtnA-Halo

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    FtnA
    

    Tags

    Halo-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p7VP1

    Plasmid

    #24726

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 7
    

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. FHUG+W (B2)

    Plasmid

    #74011

    Purpose

    lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

    Depositor

    Robert Malenka, Oliver Schluter, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_E2-Crimson

    Plasmid

    #62410

    Purpose

    MXS_chaining vector with E2-Crimson

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP-AAV-ChATen-mCherry

    Plasmid

    #193741

    Purpose

    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

    Depositor

    Hynek Wichterle

    Insert

    ChAT enhancer, mini promoter, synthetic intron, mCherry
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    1000 bp ChAT enhancer with a 32 bp minimal promot…

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid

    #174091

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Promoter

    pCMV and T7

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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