We narrowed to 16,276 results for: nes

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AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159960

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159959

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159963

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196990

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

Depositor

Elizabeth Kirby

Publication

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

VEGFR2

Use

CRISPR and Lentiviral

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTALETF_v2 (NN)

Plasmid

#32185

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NN for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLEX307-HCoV-NL63-3CL-C144A

Plasmid

#160287

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL-C143A

Plasmid

#160289

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C143A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL-C144A

Plasmid

#160285

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCSaE500

Plasmid

#53182

Purpose

Inducible expression of E gene which produces a lysis protein that induces autolysis.

Depositor

Lingchong You

Publication

Tanouchi et al Mol Syst Biol. 2012;8:626. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCSaE500

Promoter

ampR-PampC cassette

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196989

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

Depositor

Elizabeth Kirby

Publication

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Non-Target

Use

CRISPR and Lentiviral

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210739

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 3

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210737

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 1

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210738

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210740

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 4

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTALETF_v2 (NG)

Plasmid

#32186

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NG for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTALETF_v2 (HD)

Plasmid

#32188

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, HD for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

Plasmid

#198054

Purpose

All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

Depositor

Lacra Bintu

Publication

Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

Inserts

rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
3xFLAG-insert

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3G and pEF

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTALETF_v2 (NI)

Plasmid

#32187

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NI for 0.5 repeat

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS

Plasmid

#241915

Purpose

Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

Depositor

Balázs Enyedi

Publication

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS

Use

Zebrafish tol2 transgenesis

Promoter

QUASM1

Available since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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