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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
lenti SYN-FLAG-dCas9-VPR

Plasmid

#114196

Purpose

Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

Depositor

Jeremy Day

Publication

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SYN

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pEvol-pAcFRS.2.t1

Plasmid

#73544

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.2.t1
pAcFRS.2.t1

Expression

Bacterial

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Publication

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pscAAV-U6-gIl1r1-CBh-mCherry-SV40late

Plasmid

#249121

Purpose

expression of gIl1r1 and mCherry

Depositor

Matthew Krummel

Publication

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gIl1r1 (Il1r1 Mouse)

Use

AAV

Promoter

hU6

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gSCR-CBh-mCherry-SV40late

Plasmid

#249124

Purpose

expression of gSCR (scrambled, non-targeting gRNA) and mCherry

Depositor

Matthew Krummel

Publication

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gSCR (Scr Mouse)

Use

AAV

Promoter

hU6

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late

Plasmid

#249125

Purpose

expression of gTgfbr2 and mCherry

Depositor

Matthew Krummel

Publication

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2 (Tgfbr2 Mouse)

Use

AAV

Promoter

hU6

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gThy1-CBh-mCherry-SV40late

Plasmid

#249126

Purpose

expression of gThy1 and mCherry

Depositor

Matthew Krummel

Publication

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1 (Thy1 Mouse)

Use

AAV

Promoter

hU6

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for U2OS

Plasmid

#238246

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

Plasmid

#235608

Purpose

Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

Depositor

Maria Barna

Publication

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms

Use

CRISPR

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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Poliovirus 1 PV1 isolate 09_05_19 3C protease

Plasmid

#228647

Purpose

Bacterial expression of the Polio 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Polio virus 3C protease

Tags

His-SUMO

Expression

Bacterial

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain

Plasmid

#228643

Purpose

Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3

Depositor

ASAP Discovery, Frank von Delft

Insert

SARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)

Tags

His-Tev

Expression

Bacterial

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

Plasmid

#228639

Purpose

Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 33 isoform B 3C protease

Tags

His-SUMO

Expression

Bacterial

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-PlatTET-wpA-pPuroTK

Plasmid

#214125

Purpose

Gene expression by Piggybac transposase in mammalian cells

Depositor

Sho Yoshimatsu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-TET

Expression

Mammalian

Mutation

Codon-optimized for mammalian cells

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

Plasmid

#147924

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Publication

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

6xbsMS2-Malat (Malat1 Mouse)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47811-pAtAct2::Cas9-nls::tSlATPase

Plasmid

#160221

Purpose

Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

Depositor

Matias Kirst

Publication

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtACT2::Cas9-nls::tSlATPase

Use

Synthetic Biology; Moclo toolkit level 1 acceptor…

Expression

Plant

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Publication

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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