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We narrowed to 2,117 results for: Pam

Showing: 1941 - 1960 of 2117 results

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pST_PvLEA4_repeats_k5_15 (pBS0767)

Plasmid

#185248

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_15. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_15

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k5_1 (pBS0766)

Plasmid

#185247

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_14 (pBS0764)

Plasmid

#185246

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_14. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_14

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k4_1 (pBS0763)

Plasmid

#185245

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k4_1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k4_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_11 (pBS0761)

Plasmid

#185243

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_11

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k5_0 (pBS0758)

Plasmid

#185242

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k5_0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_7 (pBS0755)

Plasmid

#185241

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_1 (pBS0754)

Plasmid

#185240

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CI016_HUMAN (pBS0747)

Plasmid

#185238

Purpose

For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CI016_HUMAN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdAPOA4-44 (pBS0745)

Plasmid

#185236

Purpose

For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdAPOA4-44

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMCh-664

Plasmid

#92252

Purpose

Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-658

Plasmid

#92257

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hADM-RFP

Plasmid

#22922

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human adrenomedullin promoter driving DsRedExpress (ADM Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gEgIL

Plasmid

#11622

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E and HSV-1 glycoprotein I

Use

Tags

His

Expression

Insect

Mutation

Dicistronic vector containing HSV-1 gE (KOS strai…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
LAMP1-mCherry-FRB*

Plasmid

#207145

Purpose

Lysosome-targeted rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

LAMP1 (LAMP1 Human)

Use

Tags

FRB* (T2098L mutant of FKBP-rapamycin binding dom…

Expression

Mammalian

Mutation

Silent mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-WT

Plasmid

#114037

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Use

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPc

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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