We narrowed to 5,570 results for: donor

1. pGEMT-v5-dL5-p2a-blastR

   Plasmid

   #161981

   Purpose

   homologous donor template for endogenous tagging

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   GSlinker-V5-dL5-P2A-blastR
   

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_ZHX3_iso1

   Plasmid

   #128239

   Purpose

   Donor vector for 3' FLAG tag of human ZHX3_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZHX3_iso1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFETCh_SSX2B

   Plasmid

   #128241

   Purpose

   Donor vector for 3' FLAG tag of human SSX2B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   SSX2B Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFETCh_ZNF326_iso1

   Plasmid

   #128236

   Purpose

   Donor vector for 3' FLAG tag of human ZNF326_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZNF326_iso1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_ZBTB49_iso1

   Plasmid

   #128240

   Purpose

   Donor vector for 3' FLAG tag of human ZBTB49_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB49_iso1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-TAF15 Stop

   Plasmid

   #84895

   Purpose

   DONOR vector for Gateway cloning of TAF15

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Promoter

   none

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-TAF15 c1222t No Stop

   Plasmid

   #84900

   Purpose

   DONOR vector for Gateway cloning of TAF15 c1222t No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Mutation

   c1222t No Stop

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDONR221-TAF15 No Stop

   Plasmid

   #84896

   Purpose

   DONOR vector for Gateway cloning of TAF15 No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Mutation

   No Stop

   Promoter

   none

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-TAF15 g1172a No Stop

   Plasmid

   #84898

   Purpose

   DONOR vector for Gateway cloning of TAF15 g1172a No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Mutation

   g1172a No Stop

   Promoter

   none

   Available Since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1 EGFP-p62 HRD

    Plasmid

    #207550

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-P62
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1 EGFP-LC3B HRD

    Plasmid

    #207551

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-LC3B
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1 EGFP-GABARAPL1 HRD

    Plasmid

    #207552

    Purpose

    Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-GABARAPL1
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AICSDP-41: ATP2A2-mEGFP

    Plasmid

    #114405

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

    Depositor

    Allen Institute for Cell Science

    Insert

    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Promoter

    Promoterless

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_C0000

    Plasmid

    #91081

    Purpose

    Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1 EGFP-Sec61B

    Plasmid

    #207553

    Purpose

    Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-Sec61B
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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