We narrowed to 7,330 results for: Ank
-
Plasmid#121014PurposeMoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTP901 integrase
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_CreER-pA
Plasmid#200542Purposemini circle middle entry plasmid for CreER-pA with flanking BsaI cut sitesDepositorInsertCreER
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_memb-EGFP-pA
Plasmid#200520PurposeMini circle middle entry plasmid for membrane GFP- pA with flanking BsaI cut sitesDepositorInsertmemb-GFP
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_memb-mCherry-pA
Plasmid#200528Purposemini circle middle entry plasmid for membrane mCherry-pA with flanking BsaI cut sitesDepositorInsertmCherry
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_memb-BFP-pA
Plasmid#200537Purposemini circle middle entry plasmid for membrane BFP-pA with flanking BsaI cut sitesDepositorInsertBFP
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdadhDN-tefAO217
Plasmid#227341PurposeReplacement of the adh gene with the expression cassette of the AO217 geneDepositorInsertFlanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
UseSynthetic BiologyAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
C113m
Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C59m
Plasmid#120976PurposeMoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmScarlet
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C63m
Plasmid#120979PurposeMoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlacI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C13m
Plasmid#120960PurposeMoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCerulean
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P30m
Plasmid#120942PurposeMoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpL-lacO1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(+)MV-NSE-FlagP-F497A-3p
Plasmid#58796PurposeExpresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequenceDepositorInsertMeasles virus antigenome
TagsFlagMutationMeV-P F497APromoterT7Available SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
C79m
Plasmid#120991PurposeMoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdA antitoxin
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C27m
Plasmid#120965PurposeMoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInserttetR (no deg tags)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C98m
Plasmid#121002PurposeMoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertGp2 toxin
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C51m
Plasmid#120975PurposeMoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfYFP (has BbsI site)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C33m
Plasmid#120968PurposeMoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAiiA degradase (Bennett, no ssra tag)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
DONOR plasmid
Plasmid#236552PurposeContains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).DepositorInsertHaloTag
ExpressionMammalianAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U19m
Plasmid#121024PurposeMoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertMG-apt
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C85m
Plasmid#120996PurposeMoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma X (ECF20_992)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C91m
Plasmid#120999PurposeMoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfCFP (Chen, Bennett)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C82m
Plasmid#120993PurposeMoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRpaI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C38m
Plasmid#120971PurposeMoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 38 (RpoS)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C21m
Plasmid#120962PurposeMoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertMG-apt
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3m
Plasmid#120935PurposeMoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertJ23150
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p4m
Plasmid#120936PurposeMoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertJ23151
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(+)MV-NSE-FlagP-F497D-3p
Plasmid#58797PurposeExpresses recombinant measles virus with Flag-P-F497D gene tagged si2 (siP) target sequenceDepositorInsertMeasles virus antigenome
TagsFlagMutationMeV-P F497DPromoterT7Available SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_GFP
Plasmid#161933PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selectionDepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pEC1.2/EC1.1_spCas9_TagRFP
Plasmid#161934PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selectionDepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAH031
Plasmid#90330PurposePromoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.DepositorInsertiLOV
TagsNonePromoterNoneAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C83m
Plasmid#120994PurposeMoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpir protein required for R6K ori
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
U25m
Plasmid#121027PurposeMoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRiboJ + UTR1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T16m
Plasmid#121035PurposeMoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P55 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
T17m
Plasmid#121036PurposeMoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P11 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTUB:FLuc
Plasmid#114578PurposeConstitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integrationDepositorInsertFirefly Luciferase
UseCre/Lox and LuciferasePromoteralpha tubulin T. gondiiAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Espin-like-ARD-C-18
Plasmid#54088PurposeLocalization: Actin Bundling, Excitation: 487, Emission: 509DepositorInsertEspin-like-ARD
TagsmEmeraldExpressionMammalianMutationAnkyrin Repeat DomainPromoterCMVAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC-ME_BFP-P2A-NTRv2-pA
Plasmid#200538Purposemini circle middle entry plasmid for BFP-P2A-NTR version 2-pA with flanking BsaI cut sitesDepositorInsertBFP, NTRv2
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-mCherry-pA ORF-1 with ATG
Plasmid#200522Purposemini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (with ATG) with flanking BsaI cut sitesDepositorInsertmCherry
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-mCherry-pA ORF-2 with ATG
Plasmid#200523Purposemini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (with ATG) with flanking BsaI cut sitesDepositorInsertmCherry
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-mCherry-pA ORF-3 with ATG
Plasmid#200524Purposemini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (with ATG) with flanking BsaI cut sitesDepositorInsertmCherry
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME mCherry-P2A-NTRv2-pA
Plasmid#200532Purposemini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sitesDepositorInsertmCherry, NTRv2
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-EGFP-pA ORF-1 with ATG
Plasmid#200514Purposemini circle middle entry plasmid for 2a-eGFP-pA ORF1 (with ATG) with flanking BsaI cut sitesDepositorInserteGFP
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-EGFP-pA ORF-2 with ATG
Plasmid#200515Purposemini circle middle entry plasmid for 2a-GFP-pA ORF 2 (with ATG) with flanking BsaI cut sitesDepositorInserteGFP
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMC-ME_2a-EGFP-pA ORF-3 with ATG
Plasmid#200516Purposemini circle middle entry plasmid for 2a-GFP-pA-ORF 3 (with ATG) with flanking BsaI cut sitesDepositorInserteGFP
UseMini-goldenAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only