We narrowed to 6,508 results for: nls

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

Plasmid

#121543

Purpose

Chronos with red nucleus-targeted fluorescence for inhibitory neurons

Depositor

Matthias Prigge

Insert

blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet

Use

AAV

Promoter

dlx5/6

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-PKI nls

Plasmid

#118301

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI nls (aa 2-36)

Tags

Flag and GFP

Expression

Mammalian

Mutation

inserting 2xnls

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpG (HES824)

Plasmid

#242689

Purpose

Prime editing in mammalian cells with SpG-based PE2 prime editors

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

PE2 mutations in MMLV RT, SpG mutations in SpCas9…

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-HIS3MX6

Plasmid

#228023

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

Plasmid

#107133

Purpose

cGAL-N driver under the control of C. elegans hsp-16.41 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans hsp-16.41 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145270

Purpose

Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(53Q)-IDR (TBP Human)

Expression

Mammalian

Mutation

Q-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

Plasmid

#134333

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Efa

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

Plasmid

#134337

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Efa

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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Dronpa-Nucleus-9

Plasmid

#57290

Purpose

Localization: Nucleus, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

SV40 T-Antigen Nuclear Localization Signal (NLS)

Tags

Dronpa-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SOX2-P2A-NLS-mClover3-IRES-Puro

Plasmid

#215544

Purpose

Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

Depositor

Kai Wang

Insert

SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)

Use

CRISPR

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm]-[tNOS:BASTA:pNOS]

Plasmid

#254067

Purpose

3x YPet expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NLS KOZ c2

Plasmid

#54443

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear localization signal (NLS) of SV40 large T antigen

Use

Cloning vector

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84239

Purpose

Expresses ABA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_WT POLH

Plasmid

#221868

Purpose

Expresses WT POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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