Addgene

We narrowed to 58 results for: OFP

Showing: 1 - 20 of 58 results

Results per page:

pQE81L-KOFP7

Plasmid

#160330

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2020 May 27;68(21):58

Insert

KOFP7

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T5

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-CyOFP1

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG2.1-CyOFP1

Plasmid

#124771

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES(A7Mut)_CyOFP1_KCNQ1

Plasmid

#173162

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Insert

KCNQ1 (KCNQ1 Human)

Use

Tags

IRES2(A7Mut)-CyOFP1

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mKOFP2-CAAX

Plasmid

#75155

Purpose

Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-CyOFP1-TEV

Plasmid

#105782

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

CyOFP1-TEV

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-TEV-CyOFP1

Plasmid

#105799

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-CyOFP1

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

Enlarge
iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Use

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-miR-142

Plasmid

#117056

Purpose

Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

miR-142-3p - OFP (Mir142 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pPKm-102

Plasmid

#90493

Purpose

pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pPKm-243

Plasmid

#90506

Purpose

mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR Human CASP9 -1

Plasmid

#198419

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Mutation

Promoter

U6; CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Mutation

Promoter

U6; CMV

Availability

Academic Institutions and Nonprofits only

Enlarge