We narrowed to 6,041 results for: golden gate

1. pGoldenGreenGate-M (pGGG-M)

   Plasmid

   #165422

   Purpose

   A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Level 2 golden gate (moclo) du…

   Tags

   Expression

   Mutation

   Promoter

   Contain GG cassette and MCS with LacZ for blue/wh…

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGoldenGate-SE9

   Plasmid

   #47677

   Purpose

   Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGoldenGate-MCY2

   Plasmid

   #47679

   Purpose

   Binary vector for Arabidopsis transformation with SapI Golden Gate sites and mCherry seed selection

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGoldenGate-SE7

   Plasmid

   #47676

   Purpose

   Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and mCherry seed selection.

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-GoldenGate

   Plasmid

   #47388

   Purpose

   Expression plasmid for TALE proteins compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-GoldenGate-VP64

   Plasmid

   #47389

   Purpose

   Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   FLAG, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGoldenGreenGate-M (pGGG-M)

   Plasmid

   #165422

   Purpose

   A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Level 2 golden gate (moclo) du…

   Tags

   Expression

   Mutation

   Promoter

   Contain GG cassette and MCS with LacZ for blue/wh…

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGoldenGate-SE9

   Plasmid

   #47677

   Purpose

   Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGoldenGate-MCY2

    Plasmid

    #47679

    Purpose

    Binary vector for Arabidopsis transformation with SapI Golden Gate sites and mCherry seed selection

    Depositor

    Jose Dinneny

    Article

    Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

    Type

    Empty backbone

    Use

    Arabidopsis transformation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGoldenGate-SE7

    Plasmid

    #47676

    Purpose

    Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and mCherry seed selection.

    Depositor

    Jose Dinneny

    Article

    Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

    Type

    Empty backbone

    Use

    Arabidopsis transformation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-GoldenGate

    Plasmid

    #47388

    Purpose

    Expression plasmid for TALE proteins compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid

    #172506

    Purpose

    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 bacterial expression vector with golden gate site for guides
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

    Plasmid

    #172514

    Purpose

    Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid

    #172508

    Purpose

    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge