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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42K

Plasmid

#194523

Purpose

High copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K

Plasmid

#194522

Purpose

Low copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N

Plasmid

#194524

Purpose

Low copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42N

Plasmid

#194525

Purpose

High copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41H

Plasmid

#194526

Purpose

Low copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H

Plasmid

#194527

Purpose

High copy vector backbone with hygromycin B selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr

Plasmid

#194528

Purpose

Low copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42K-TDH3pr

Plasmid

#194529

Purpose

High copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41N-TDH3pr

Plasmid

#194530

Purpose

Low copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42N-TDH3pr

Plasmid

#194531

Purpose

High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41H-TDH3pr

Plasmid

#194532

Purpose

Low copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H-TDH3pr

Plasmid

#194533

Purpose

High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41K-TDH3pr-mNeon

Plasmid

#194534

Purpose

Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42K-TDH3pr-mNeon

Plasmid

#194535

Purpose

High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42N-TDH3pr-mNeon

Plasmid

#194537

Purpose

High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41H-TDH3pr-mNeon

Plasmid

#194538

Purpose

Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-hNIL-BSD-mApple

Plasmid

#124230

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Insert

NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge