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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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32,401 results

Showing: 4141 - 4160 of 32401 results

  3. p405TDH3

    Plasmid
    #15969
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405TDH3 (TDH3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15969-plasmid-map-sequence-id-8454

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  7. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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  10. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  16. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pSR47S Cm

    Plasmid
    #41892
    Depositor
    Howard Steinman
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Expression
    Bacterial
    Available Since
    May 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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  20. pET-22b DT 51E/148K

    Plasmid
    #11081
    Depositor
    John Collier
    Insert
    DT
    Tags
    His
    Expression
    Bacterial
    Mutation
    51E/148K, catalytically inactive
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11081-plasmid-map-sequence-id-306912

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Showing: 4141 - 4160 of 32401 results