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Plasmid#60854PurposeExpresses firefly luciferaseDepositorInsertFirefly luciferase (optimized for Mycobacteria)
UseTagsExpressionBacterialMutationcodon optimized for MycobacteriaPromoterhsp60Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB223
Plasmid#164127PurposeNew measurement standardDepositorInsertYFP-PP7
UseTagsExpressionBacterialMutationPromoterJ23101Available sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP2
Plasmid#69628PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYAB
Plasmid#217670PurposeFor expression of SUMO tagged human alpha B crystallinDepositorInsertCRYAB (CRYAB Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorInsertGα12-LgB115 (GNA12 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPclpBAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD565
Plasmid#153027PurposedxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNADepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn E46K
Plasmid#105728PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein E46K (SNCA Human)
UseTagsExpressionBacterialMutationE46KPromoterT7Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
strep-IRF8
Plasmid#102872PurposeExpress strep-tagged mouse IRF8 protein in E. coliDepositorInsertIRF8 (Irf8 Mouse)
UseTagsstrep tagExpressionBacterialMutationPromoterT7Available sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB6k-GFP
Plasmid#35357DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pRSETB
Plasmid#51960Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagseGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/X
Plasmid#168017PurposeExpresses BoNT/X-LC(1-439)-6xHis in bacterial cellsDepositorInsertBoNT/X-LC(1-439)-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK131
Plasmid#71548PurposeProduces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)DepositorInsertAlanine racemase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTi4.0_SpdCas9_pmcDA1
Plasmid#204613PurposeCRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticumDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP
Plasmid#166443PurposeBacterial expression of 6x His tagged YAPDepositorInsertYAP (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only