31,800 results
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Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
TagsmKate2ExpressionBacterialAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK1008 (pSL7)
Plasmid#191003PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed trigger
Plasmid#107357PurposeTrigger sequence for pNP1 eforRed toehold switchDepositorInserttoehold trigger 1
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCpf1-sp
Plasmid#122186PurposeBacterial genome editing plasmid with spectinomycin resistance markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EcAvs4
Plasmid#188794PurposeAvs4 from Escherichia coli NCTC11132DepositorInsertEcAvs4
ExpressionBacterialAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialPromoterpPH (Polyhedrin promoter)Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN1201
Plasmid#74695PurposeCircuit backbone plasmid (LacZa insert)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
Tags6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_flag
Plasmid#210404PurposeFor expression of VHH with C-terminal his and Flag tagsDepositorTypeEmpty backboneTagshis6-flagExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET32a-LIC
Plasmid#62310PurposeE. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEVDepositorTypeEmpty backboneTags6xHis, S-tag, and Trx-TagExpressionBacterialPromoterT7-lacOAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB208
Plasmid#164123PurposePtac driving PP7 repeatsDepositorInsertYFP-PP7
ExpressionBacterialPromoterPtacAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB035
Plasmid#181944PurposeaTc-inducible expression of mNeonGreenDepositorInsertmNeonGreen; tetR (AX04_RS23485 Synthetic)
ExpressionBacterialPromotermNG-PLtetO-1; tetR-J23106Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK24] pACYCDuet His-c7orf59|HBXIP
Plasmid#136143Purposebacterial expression of c7orf59 and HBXIPDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…Available SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only