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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL723

Plasmid

#140393

Purpose

Expresses HucR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-HucR-T

Tags

6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 M(272-617)

Plasmid

#22482

Purpose

Please note this plasmid contains GST-Hsp90 residues 273-617.

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha M domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA

Plasmid

#48884

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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NRAS

Plasmid

#55654

Purpose

expression of mouse NRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NRAS (Nras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
PB6K-mcherry-iATPsnFR1.1

Plasmid

#227239

Purpose

Inducible ATP sensor for Pseudomonas putida

Depositor

Fuzhong Zhang

Article

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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g5s pD42

Plasmid

#117452

Purpose

Expression of Cyth2

Depositor

Richard Kahn

Insert

Cyth2 (CYTH2 Human)

Tags

V5-His6

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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RRAS

Plasmid

#38815

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RRAS (Rras Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pBZS-YP_001497877

Plasmid

#198366

Purpose

Express YP_001497877 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001497877 (B681L )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698N

Plasmid

#92242

Purpose

Express H698N mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698N mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Asparagine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMS31: pTwinStrep-SUMO-PmcTnsAB

Plasmid

#168164

Purpose

PmcTnsAB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST TnsAB

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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mT-Sapphire-pBAD

Plasmid

#54571

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

6xHis and mT-Sapphire

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pdestDD04Neo

Plasmid

#26386

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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