30,810 results

1. NLS-tdTomato

   Plasmid

   #163443

   Purpose

   Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone

   Depositor

   Sanford Simon

   Insert

   tdTomato
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptRNAfMet-A37

   Plasmid

   #196657

   Purpose

   Plasmid for overexpression of native initiator tRNA (A37)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37 expression cassette
   

   Expression

   Bacterial

   Promoter

   metY native promoter

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL0915 (CRISPR with target 3)

   Plasmid

   #130643

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(target-3)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAtetO_6xHis-v5.9

   Plasmid

   #155259

   Purpose

   Plasmid for the aTc-inducible expression of the v5.9

   Depositor

   Andrew Ellington

   Article

   Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

   Insert

   v5.9
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   tet PA

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-41-4GS-LaG-2

   Plasmid

   #172766

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41-4GS-LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T Crkl

   Plasmid

   #36400

   Depositor

   Nora Heisterkamp

   Article

   ten Hoeve et al Cancer Res. 1994 May 15;54(10):2563-7.

   Insert

   Crkl (CRKL Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pJH474_pTech_Para-supP

   Plasmid

   #163718

   Purpose

   Arabinose-inducible expression of supP

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   supP
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFosill-3

   Plasmid

   #89697

   Purpose

   to make Fosmids that can be converted into Illumina sequencable libraries

   Depositor

   Andreas Gnirke

   Article

   Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJS008

   Plasmid

   #188331

   Purpose

   PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::wrmScarlet::mIAA7
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_Nsp9

    Plasmid

    #156478

    Purpose

    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFG036

    Plasmid

    #137996

    Purpose

    cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    cI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Thermosensitive

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJKR-H-ttgR

    Plasmid

    #62565

    Purpose

    Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. YWHAG

    Plasmid

    #36903

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    YWHAG (YWHAG Human)
    

    Tags

    6xHis with TEV Cleavage

    Expression

    Bacterial

    Available Since

    June 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. ETV2_pet28a

    Plasmid

    #131665

    Purpose

    Bacterial expression of ETS gene ETV2

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV2 (ETV2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSty11

    Plasmid

    #12401

    Depositor

    Titia de Lange

    Article

    de Lange et al EMBO J. 1992 Feb . 11(2):717-24.

    Insert

    Sty11
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-SUMO-AsCas12f-2NLS

    Plasmid

    #171613

    Purpose

    NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    NLS-AsCas12f1-NLS
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-c2E

    Plasmid

    #75291

    Purpose

    Expresses proteins in the cytoplasm as fusions to maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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