32,662 results
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Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUS212
Plasmid#84580PurposeE.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 21, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF18
Plasmid#222593PurposeBB2 ZWF1 tet-on overexpression cassetteDepositorArticleInsertZWF1
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromotertet-onAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglB
Plasmid#134676PurposeExpresses His6-TglBin E. ColiDepositorInsertTglB
TagsHis6ExpressionBacterialPromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 hBad
Plasmid#35567DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT006-GCN4-HaloPM
Plasmid#163386PurposeTo express 4X sGCN4 module in the G-baToN systemDepositorInsert4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOKD5
Plasmid#17215DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAT2.1
Plasmid#35620DepositorTypeEmpty backboneTags6xHIS and TATExpressionBacterialPromoterT7Available SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK647
Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
ExpressionBacterialPromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP3
Plasmid#69650PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam
ExpressionBacterialPromoterE. coli PBADAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Vinculin tail
Plasmid#107154Purposebacterial expressionDepositorAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Kindlin
Plasmid#186135PurposeFor expression of recombinant kindlin in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VASP_WH1
Plasmid#109790PurposeProtein expression and purification of VASP_WH1DepositorInsertVASP_WH1 (VASP Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyExpressionBacterialPromoterpLlacO-1 for LuxR, pLux for GFPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only