30,176 results
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Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEND-Lx
Plasmid#25010DepositorInsertpMEND-Lx
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsUseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
NylB'-SCY_pET21b
Plasmid#215417PurposeExpression construct for NylB'-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB'-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
UseTagsHis tag, FLAG tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY10
Plasmid#24664DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pET21-Drp1
Plasmid#72927PurposeBacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1 (Dnm1l Mouse)
UseTags6xHis-tag and PreScission Protease siteExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetR193-Wasabi
Plasmid#86105PurposemWasabi reporter gene gated by temperature-sensitive TetR I193NDepositorInsertsTetRts
mWasabi
UseTags6xHisExpressionBacterialMutationI193NPromoterptetA and ptetRAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
UseTags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB038a
Plasmid#99211PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
UseTagsExpressionBacterialMutationamber codons at positions 29 and 184 of gIIIPromoterPpsp and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-hKdm2b-CxxC-PHD
Plasmid#49216PurposeBacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.DepositorInsertKdm2b (KDM2B Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GRvT
Plasmid#87363PurposeExpresses GRvT, a tandem heterodimer green and red fluorescent proteinDepositorInsertGRvT
UseTagsHis-TagExpressionBacterialMutationPromoterAraBadAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pXWJ109As(RS-te-AAV)
Plasmid#123156PurposeFor TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfYFPBb
Plasmid#118233PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFPDepositorInsertflagellin promoter driven msfYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only