30,235 results

1. pCri_L830-antiSH2-OptoMB-V416L

   Plasmid

   #159936

   Purpose

   Opto-MB bacterial expression

   Depositor

   Jared Toettcher, Jose Avalos

   Article

   Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

   Insert

   anti-SH2 MB with SS58 insert
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AsLOV2 V416L

   Promoter

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCri_L889-antiSH2-OptoMB-Triple

   Plasmid

   #159937

   Purpose

   Opto-MB bacterial expression

   Depositor

   Jared Toettcher, Jose Avalos

   Article

   Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

   Insert

   anti-SH2 MB with SS58 insert
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AsLOV2 V416I_G528A_N538E

   Promoter

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTAMAHISTEV_PfeAQ482A

   Plasmid

   #128945

   Purpose

   expresses Q482A mutant of PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75), …

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHL 1075

   Plasmid

   #53004

   Purpose

   pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   fhlA
   sodB
   

   Use

   Synthetic Biology

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   contains −309 to +96 bp relative to start codon a…

   Promoter

   pCon and pLlacO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. APneg-SNAP25d

   Plasmid

   #168014

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   SNAP25-T7PAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQTEV-YWHAE

   Plasmid

   #31562

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSUN5

   Plasmid

   #25491

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NSUN5 (NSUN5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. PET28a-cpLumiLuc-Rapamycin sensor

   Plasmid

   #186292

   Purpose

   Expression Rapamycin sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-Rapamycin sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T1-TRAF6-C70A

   Plasmid

   #43930

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   TNF Receptor-Associated Factor 6 (Traf6 )
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C70A

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGC_RT_XVE

    Plasmid

    #136970

    Purpose

    Goldengate/Greengate module for generation of beta-estradiole inducible genes.

    Depositor

    Klaus Theres

    Insert

    XVE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication 834 T > A,

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23-CBD-(-PP)-EGFP

    Plasmid

    #12597

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    Cdc42 Biosensor (WAS Human)
    

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    F271C

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5KKP (PUS7)

    Plasmid

    #101878

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PUS7 (PUS7 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET-51b(+)_NADP-Snifit

    Plasmid

    #113913

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E2F-1 wt-pGex2TK

    Plasmid

    #21668

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    E2F-1 (E2F1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. RFP-(SIM)1

    Plasmid

    #127094

    Purpose

    Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD/HisB-Slow-FT

    Plasmid

    #31918

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNR291

    Plasmid

    #79555

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    GFPmut3b
    mTagBFP
    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PTP1B-1-401

    Plasmid

    #78683

    Purpose

    bacterial expression of PTP1B aa 1-401

    Depositor

    Eli Chapman

    Insert

    PTPN1 (PTPN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta 402-435

    Promoter

    araBAD

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPEPX

    Plasmid

    #122631

    Purpose

    BglFusion compatible integration vector for Streptococcus pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MECR

    Plasmid

    #38850

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MECR (Mecr Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only