Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,126 results

Showing: 8341 - 8360 of 32126 results

  5. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

    Enlarge

  6. pPLP4HS-mCf

    Plasmid
    #229988
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6 and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229988-plasmid-map-sequence-id-458286

    Enlarge

  7. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229984-plasmid-map-sequence-id-458288

    Enlarge

  8. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

    Enlarge

  9. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    Strep-tag II
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229986-plasmid-map-sequence-id-458306

    Enlarge

  10. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229987-plasmid-map-sequence-id-458283

    Enlarge

  11. pGEX4T-3-6AOptParkin

    Plasmid
    #165107
    Purpose
    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene
    Depositor
    Arthur Haas
    Insert
    Parkin isoform 1 (PRKN Human)
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165107-plasmid-map-sequence-id-326710

    Enlarge

  12. NUDT1

    Plasmid
    #74660
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT1 (NUDT1 Human)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pBS-AMVpl-γWT-nAchR

    Plasmid
    #97187
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97187-plasmid-map-sequence-id-183032

    Enlarge

  16. ARF1 (ARF1A-c001)

    Plasmid
    #110258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ARF1 (ARF1 Human)
    Tags
    StrepII-TEV
    Expression
    Bacterial
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110258-plasmid-map-sequence-id-212329

    Enlarge

  18. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

    Enlarge

  19. pHYP-NPC-10H

    Plasmid
    #162789
    Purpose
    SARS-CoV-2 Nucleocapsid Protein expression in E.coli
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771
    Insert
    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    10xHis
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162789-plasmid-map-sequence-id-333793

    Enlarge

Showing: 8341 - 8360 of 32126 results