30,875 results

1. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE060

   Plasmid

   #176817

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   611
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPSF5A

   Plasmid

   #42353

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CPSF5A (NUDT21 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pBbS1c-RFP

   Plasmid

   #35338

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Trc

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEW3: pET-32a Dot1L(2-416)

   Plasmid

   #124098

   Purpose

   Dot1L(2-416) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-416) (DOT1L Human)
   

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD077

   Plasmid

   #136473

   Purpose

   SSB-mTur2 IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-mTur2 (ssb E. coli)
   

   Tags

   mTurquoise2

   Expression

   Bacterial

   Mutation

   mTurquoise2 inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. drebrin-His

   Plasmid

   #40363

   Depositor

   Phillip Gordon-Weeks

   Article

   Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

   Insert

   drebrin E2 (DBN1 Human)
   

   Tags

   His and T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

   Plasmid

   #183069

   Purpose

   Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   CRISPR

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   Wild-type

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBR6k-GFPuv

    Plasmid

    #106384

    Purpose

    IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDO23A

    Plasmid

    #27367

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    pUC/ori, bla
    

    Use

    Gateway donor vector

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. ESCRT-II

    Plasmid

    #17633

    Depositor

    James Hurley

    Article

    Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

    Insert

    pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSG291

    Plasmid

    #115605

    Purpose

    surface-displayed Null, used in composability experiments; low copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH6HTC_MANF-Halo

    Plasmid

    #175341

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    MANF (MANF Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1alpha-mClover3-bGHpA

    Plasmid

    #172286

    Purpose

    Constitutive expression of mClover3

    Depositor

    Patrik Verstreken

    Insert

    mClover3
    

    Expression

    Bacterial

    Promoter

    EF1alpha

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBL725

    Plasmid

    #140395

    Purpose

    Expresses SmtB protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-SmtB-T
    

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK90

    Plasmid

    #13323

    Depositor

    Martin Gellert

    Article

    Mizuuchi et al J Biol Chem. 1984 Jul 25. 259(14):9199-2

    Insert

    gyrA
    

    Expression

    Bacterial

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42

    Plasmid

    #153525

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#42

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#42
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMyCA-mCherry

    Plasmid

    #84272

    Purpose

    mCherry fluorescence protein expression using M. smegmatis

    Depositor

    Young-Hwa Song

    Article

    Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

    Insert

    mCherry
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    Acetamidase

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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