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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-CImf

Plasmid

#40126

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lac inducible lambda repressor

Use

Synthetic Biology

Tags

mf-lon ssrA tag

Expression

Bacterial

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_Nef_MCS2

Plasmid

#66079

Purpose

Single vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Insert

Nef

Tags

6xHis and HRV 3C Site

Expression

Bacterial

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NfPolyA_pET21b

Plasmid

#215413

Purpose

Expression construct for NfPolyA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NfPolyA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNS207

Plasmid

#35041

Depositor

Stephen Sligar

Article

Nikkila et al Eur J Biochem. 1991 Dec 5;202(2):309-13.

Insert

cytochrome b562

Expression

Bacterial

Promoter

lac

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pE6c

Plasmid

#17466

Purpose

modified pENTR vector for C-terminal EYFP tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

EYFP

Expression

Bacterial, Insect, Mammalia…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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enz.083-NMT1

Plasmid

#154047

Purpose

Expresses NMT1 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

NMT1 (N-myristoyltransferase 1) (NMT1 Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSU315

Plasmid

#169697

Purpose

Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

Depositor

Nara Figueroa

Article

Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

Insert

1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene

Tags

1xHA tag

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJH474_pTech_Para-supP

Plasmid

#163718

Purpose

Arabinose-inducible expression of supP

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Tags

His

Expression

Bacterial

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMAL-c2E

Plasmid

#75291

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pQTEV-CDO1

Plasmid

#31292

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cysteine dioxygenase, type I (CDO1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pUMV19

Plasmid

#67161

Purpose

inducible operation of the mevalonate pathway in E. coli

Depositor

Tomohisa Kuzuyama

Article

Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

Insert

the mevalonate pathway genes

Expression

Bacterial

Promoter

lacZ

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

Plasmid

#169077

Purpose

Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GFP tag, GGlinker, TEV cleavage site. StrepII and…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-dTomato

Plasmid

#117391

Purpose

Tn7 tagging vector pTn7xTS with dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pRSETa-mMaple3-V157I

Plasmid

#98577

Purpose

photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mMaple3-V157I

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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