30,875 results

1. pJK210

   Plasmid

   #73325

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis protein VioD

   Depositor

   T. J. Kappock

   Insert

   violacein biosynthesis protein VioD
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-CohIII-CBM-HIS-LPETGG

   Plasmid

   #91697

   Purpose

   CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   CohIII-CBM-HIS-LPETGG
   

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P2-MDMX (23-111)

   Plasmid

   #62064

   Purpose

   Expression of human MDM4 (residues 23-111) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 23-111

   Promoter

   tac

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. His6-Twin-Strep-SUMO-pET28a-EfaCas2

   Plasmid

   #112794

   Purpose

   To express enterococcus faecalis Cas2 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas2
   

   Tags

   His6-Twin-Strep-SUMO

   Expression

   Bacterial

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx Rab1A(S20C) CL

   Plasmid

   #224272

   Purpose

   Protein purification of Rab1A (S20C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rab1A (RAB1A Human)
   

   Expression

   Bacterial

   Mutation

   S20C

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLY071f

   Plasmid

   #227942

   Purpose

   SICLOPPS precursor for cyclo-CRCVFSG

   Depositor

   Tina Wang

   Article

   Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

   Insert

   Evolved Ssp SICLOPPS-CRCVFSG
   

   Expression

   Bacterial

   Mutation

   Evolved Ssp split inteins with multiple mutations…

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Tags

   6x His

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SSH-pSUMO-α-COPI-WD40

   Plasmid

   #208341

   Purpose

   Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
   

   Tags

   His-tag, SUMO-tag, and Strep-tag

   Expression

   Bacterial

   Mutation

   Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad33_ISEc41_reRNA

   Plasmid

   #205341

   Purpose

   Bacterial expression of ISEc41 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISEc41 TnpB
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad33_ISEc46_reRNA

    Plasmid

    #205343

    Purpose

    Bacterial expression of ISEc46 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc46 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBad33_ISNsp2_reRNA

    Plasmid

    #205350

    Purpose

    Bacterial expression of ISNsp2 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISNsp2 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_SKAP2-1/1

    Plasmid

    #91447

    Purpose

    Protein expression and purification of human SH3 domain construct SKAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SKAP2-1/1 (SKAP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEE050

    Plasmid

    #176807

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    601
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB18cmBFP1A5

    Plasmid

    #14035

    Depositor

    Patrick Daugherty

    Article

    Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

    Insert

    BFP1A5
    

    Use

    Yeast/bacteria optimized codons

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBluescript GPX-1

    Plasmid

    #13038

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

    Insert

    GPX-1 (GPX1 Human)
    

    Expression

    Bacterial

    Mutation

    Additional GCG upstream of codon 11. Codon 92 is …

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pE4n

    Plasmid

    #17458

    Purpose

    modified pENTR vector for N-terminal GST tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    GST and Thrombin site

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLS1

    Plasmid

    #31490

    Depositor

    Kathleen Matthews

    Article

    Swint-Kruse et al Protein Sci. 2001 Feb . 10(2):262-76.

    Insert

    lactose repressor protein (lacI )
    

    Expression

    Bacterial

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pBSV2G_PflaB-mCeruleanBb

    Plasmid

    #118235

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven mCerulean
    

    Expression

    Bacterial

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AlphaC

    Plasmid

    #124525

    Purpose

    pColE1_AlphaC

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIC-CTH10STII-GG

    Plasmid

    #215804

    Purpose

    Cloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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