33,526 results

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Wild-type-ThermoCas9-3xNLS

Plasmid

#254685

Purpose

Expresses Wild-type ThermoCas9 with 3x NLS tags. Utilized for RNP transfection

Depositor

Hong Li

Publication

Roth et al Nature. 2026 Apr 15. doi: 10.1038/s41586

Insert

Wildtype ThermoCas9

Tags

NLS, SV40 NLS, and nucleoplasmin NLS

Expression

Bacterial

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLann-HRV-3C

Plasmid

#254756

Purpose

Express Prescission Protease

Depositor

Brian Kuhlman

Publication

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

HRV-3C

Tags

GST

Expression

Bacterial

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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MultiCAST-miniTn-Kan

Plasmid

#248974

Purpose

MultiCAST mini-Tn donor plasmid containing kanamycin selectable marker

Depositor

Matthew Waldor

Publication

Basta et al bioRxiv [Preprint]. 2025 Nov 1:2025.10.3

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pZB246-T7-T+

Plasmid

#250903

Purpose

Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone

Depositor

Timothy Whitehead

Publication

Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.

Insert

Thermally Stable T7 RNA Polymerase

Tags

6xHis

Expression

Bacterial

Mutation

Arginine 31 to Aspartic Acid, Alanine 61 to Argin…

Promoter

pT5-lacUV

Available since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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CHIKV eGFP-Zeo replicon

Plasmid

#249289

Purpose

SP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassette

Depositor

Amy Kistler

Insert

CHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes

Expression

Bacterial

Mutation

structural polyprotein open reading frame deleted…

Promoter

SP6

Available since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD-ColH-HylB

Plasmid

#248631

Purpose

expresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coli

Depositor

Edwin Manuel

Publication

Avsharian et al Biomolecules. 2024 Nov 17;14(11):1458. d

Insert

Myc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB

Tags

His and Myc

Expression

Bacterial

Promoter

araBAD

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0001

Plasmid

#232210

Purpose

GGA cloning M-AGGA-promoter-ccdb-TTCC-SNAC-HIS

Depositor

David Baker

Publication

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Publication

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-pvc10-Cas9

Plasmid

#243755

Purpose

For affinity purification of Pvc10 fused to Cas9

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10-Cas9

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9

Plasmid

#243744

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_HUHe-Pvc10-Cas9

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS9_10xHis_MBP_TEV_dCas9

Plasmid

#244831

Purpose

Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/H840A

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Publication

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-Pls1-sfGFP

Plasmid

#205748

Purpose

Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.

Depositor

Jerome Bonnet

Publication

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls1 (Phyperspank mutant, IPTG inducible)

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_Cas9d_Plasmid

Plasmid

#239445

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Publication

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

Cas9d

Expression

Bacterial

Promoter

T7

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14112 tyrosinase-targeting sgRNA

Plasmid

#239751

Purpose

guide for tyrosinase

Depositor

Stanley Qi

Publication

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting tyrosinase

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ14103 aeBlue-targeting_sgRNA

Plasmid

#239750

Purpose

guide for aeBlue

Depositor

Stanley Qi

Publication

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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p300dAIL KAT LIC 1M

Plasmid

#233588

Purpose

Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

Depositor

Scott Showalter

Publication

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300dAIL KAT (EP300 Human)

Tags

6xHis MBP

Expression

Bacterial

Mutation

deleted amino acids 1523-1554

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTW386c

Plasmid

#237321

Purpose

expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Publication

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS644

Plasmid

#238209

Purpose

Expresses native FpTasA TIGR locus. CmR

Depositor

Feng Zhang

Publication

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

FpTasA

Expression

Bacterial

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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