34,239 results
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Plasmid#225614PurposeReplicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.DepositorInsertmCerulean
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCN38-kimA-yfp
Plasmid#227635Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGMβ1
Plasmid#216202PurposeChromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.DepositorTypeEmpty backboneUseShuttle vector, conjugal plasmid, theta type rep…ExpressionBacterialPromoterlac promoter in pGEM-Teasy, Promoters in pAMbeta1…Available sinceJan. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
Tags6xHISExpressionBacterialPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag and Histidine tagExpressionBacterialPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Para
Plasmid#218362PurposeEmpty backbone with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-EV
Plasmid#202466PurposeEmpty vector of pSMART-HCKanDepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-Myc-MBP-HA
Plasmid#222711PurposeExpression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cellsDepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-HIS-FLAG-AtUBQ11
Plasmid#222720PurposeExpression HIS-FLAG-AtUBQ11 in in bacterial cellsDepositorAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-dCas9
Plasmid#220196Purposemini-Tn7 dCas9 expression vector - XylS/pM promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterXylS/pMAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorPublicationInsertsP gene
M gene
ExpressionBacterialPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pProEx Rac1(G12C) CL
Plasmid#224269PurposeProtein purification of Rac1 (G12C)DepositorAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO1
Plasmid#209975PurposeContains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable sinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDM026
Plasmid#216808PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only