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31,828 results

Showing: 8561 - 8580 of 31828 results

  1. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    PgyrA
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  6. PARP2A

    Plasmid
    #42450
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP2A (PARP2 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pGEX-2T-Rac1-T17N

    Plasmid
    #12978
    Depositor
    Gary Bokoch
    Insert
    Rac1 DN (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  8. pE4n

    Plasmid
    #17458
    Purpose
    modified pENTR vector for N-terminal GST tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    GST and Thrombin site
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17458-plasmid-map-sequence-id-8626

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  9. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  10. CFL1 (CFL1A-c002)

    Plasmid
    #98237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CFL1A (CFL1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00241:Cofilin_ADF 27-148
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98237-plasmid-map-sequence-id-193416

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  12. pRS415-SPT6

    Plasmid
    #10993
    Depositor
    Francisco Malagon
    Insert
    SPT6 (SPT6 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Available Since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10993-plasmid-map-sequence-id-6828

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  14. 6VAH

    Plasmid
    #162273
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    TEAD2 (TEAD2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162273-plasmid-map-sequence-id-319837

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  16. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  17. pcDNA3.1.H1-NP

    Plasmid
    #134367
    Purpose
    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    H1-NP (HRH1 Human)
    Tags
    Flag and natural peptide of nanoluciferase
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134367-plasmid-map-sequence-id-306386

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  18. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  19. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid
    #101217
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101217-plasmid-map-sequence-id-197215

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Showing: 8561 - 8580 of 31828 results