30,877 results

1. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217767

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400

   Promoter

   T7 promoter

   Available Since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

   Plasmid

   #217766

   Purpose

   Fluorescent reporter for ceramide (for bacterial expression and purification)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   T7 promoter

   Available Since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSC101-GFPmut2-mScarlet-I TAG

   Plasmid

   #208188

   Purpose

   nonsense mistranslation, TAG stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Insertion 6TAG*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC101-GFPmut2-mScarlet-I TGA

   Plasmid

   #208189

   Purpose

   nonsense mistranslation, TGA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC101-GFPmut2-mScarlet-I TGATC

   Plasmid

   #208190

   Purpose

   nonsense mistranslation, TGA stop variant 2

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7TCT(Ser)

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC101-GFPmut2-mScarlet-I T7

   Plasmid

   #208191

   Purpose

   frameshift mistranslation, T7

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6TTTTTTT

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSC101-GFPmut2-mScarlet-I A5

   Plasmid

   #208192

   Purpose

   frameshift mistranslation, A5

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAA

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC101-GFPmut2-mScarlet-I A4

   Plasmid

   #208193

   Purpose

   frameshift mistranslation, A4

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAA

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC101-GFPmut2-mScarlet-I A6T

   Plasmid

   #208194

   Purpose

   frameshift mistranslation, A6T

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAAAT

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC101-GFPmut2-mScarlet-I C3T

    Plasmid

    #208195

    Purpose

    frameshift mistranslation, C3T

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6CCCT

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC101-GFPmut2(ΔmScarlet)

    Plasmid

    #208185

    Purpose

    GFP positive, mScarlet-I negative control

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Insert

    GFPmut2
    

    Use

    Reporter

    Expression

    Bacterial

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC101-GFPmut2-mScarlet-I 6Trp7Ser

    Plasmid

    #208186

    Purpose

    mistranslation GFP and mScarlet-I positive control with Trp insertion

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6Trp7Ser

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL-c4E

    Plasmid

    #75293

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCPP5537

    Plasmid

    #128730

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    HopAO1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNAS1B split mCherry N-link/link-C

    Plasmid

    #61965

    Purpose

    Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-link
    link-C-mCherry
    

    Expression

    Bacterial

    Promoter

    PBAD and PLtetO

    Available Since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMD5

    Plasmid

    #138652

    Purpose

    phiBT1 integrating Streptomyces expression vector

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEB2-2ndVal mKate2

    Plasmid

    #104030

    Purpose

    Plasmid encoding 2ndVal mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal mKate2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    mKate2 with valine inserted in 2nd position

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Twitch-1 pRSETB

    Plasmid

    #48207

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

    Insert

    Twitch-1
    

    Tags

    6xHIS, ECFP, and cpCit174

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCDH-EF1a-mTagBFP-T2A-TetOn3G

    Plasmid

    #179888

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    mTagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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