30,238 results

1. pETM10 with rat FL Annexin A11

   Plasmid

   #164496

   Purpose

   For bacterial expression of rat His-tagged FL Annexin A11

   Depositor

   Anni Vedeler

   Article

   Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

   Insert

   rat FL Annexin A11 (Anxa11 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJKW0776

   Plasmid

   #119024

   Purpose

   E.coli expression plasmid for PmSPS2

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmSPS2
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN4023

   Plasmid

   #74701

   Purpose

   Output plasmid: PBM3R1-YFP

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PBM3R1-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP S2TAG

   Plasmid

   #68296

   Purpose

   S2TAG GFP reporter

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   GFP S2TAG
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ Integrin beta 1b-mKate

   Plasmid

   #105975

   Purpose

   in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Integrinbeta1b
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF005-sgRNA-shuffle-in

   Plasmid

   #104441

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF004-sgRNA-shuffle-in

   Plasmid

   #104440

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS hTSP-3

    Plasmid

    #12995

    Depositor

    Jack Lawler

    Insert

    TSP-3 (THBS3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pPBO.ACT005

    Plasmid

    #182715

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5VBD (USP09X)

    Plasmid

    #101260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP09X (USP9X Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. rpoDs366C

    Plasmid

    #129688

    Purpose

    Expression of Sigma 70 S366C

    Depositor

    Jeff Gelles

    Article

    Mumm et al bioRxiv 2020

    Insert

    rpoD (rpoD E. coli)
    

    Use

    Tags

    His6/thrombin

    Expression

    Bacterial

    Mutation

    C132S C291S C295S S366C

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiex-1 AC30_eGFP

    Plasmid

    #82715

    Purpose

    Expresses a GFP-tagged recombinant adenylyl cyclase.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPath-pR-integrase

    Plasmid

    #200000

    Purpose

    Entry vector for biosynthetic pathway. pR-phiC31 integrase

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ftsZ-RFP

    Plasmid

    #63842

    Purpose

    This plasmid is designed as a bacterial cell division sensor

    Depositor

    Howard Salis

    Insert

    ftsZ, mRFP1 and GFPmut3b
    

    Use

    Tags

    mRFP1

    Expression

    Bacterial

    Mutation

    Gene is translationally fused to RFP

    Promoter

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET FLIPE intramol-1u

    Plasmid

    #13549

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol WT (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_NR6A1_Hormone-recep

    Plasmid

    #109945

    Purpose

    Protein expression and purification of NR6A1_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR6A1_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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