32,129 results

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pCis_2+7+8+5

Plasmid

#60589

Purpose

a 4-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+7+8+5

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Expression

Bacterial

Promoter

PlacIq and Ptaq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP

Plasmid

#185013

Purpose

Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

Sirius-GFP

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a D832A

Plasmid

#113517

Purpose

Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

D832A

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pIA1362

Plasmid

#166860

Purpose

Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp7 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJJS011

Plasmid

#188334

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mTagBFP2::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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UBC32

Plasmid

#25213

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubc32.001.158 (UBE2K Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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PDLIM4_PDZ_1

Plasmid

#103941

Purpose

Protein expression and purification of RIL BGLII PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDLIM4 (PDLIM4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_B

Plasmid

#145950

Purpose

Bacterial Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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MT1X

Plasmid

#105709

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1x

Depositor

Artur Krężel

Insert

Metallothionein 1x (MT1X Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT1F

Plasmid

#105703

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1f

Depositor

Artur Krężel

Insert

Metallothionein 1f (MT1F Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ-mNeongreen

Plasmid

#182392

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-LZ-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPb2Cas12a

Plasmid

#174686

Purpose

Expresses 6xHis-MBP tagged Pb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRibo EcoHind

Plasmid

#36252

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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