31,828 results
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Plasmid#186785PurposeC-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
TagsHis tagExpressionBacterialMutationdeleted amino acids 503-512Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (carbR)
Plasmid#213911PurposeATc-inducible himar1C9 transposase expression - carb/amp resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
drebrin-His
Plasmid#40363DepositorAvailable SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKSS
Plasmid#39272DepositorTypeEmpty backboneUsePositive-selection cloning vectorExpressionBacterialAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSAN-QUE20C
Plasmid#129316PurposeQUEEN ATP sensor for 20degCDepositorInsertQUEEN-20C
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PAK2 wt
Plasmid#31671DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
ExpressionBacterialPromoterPR promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYH74
Plasmid#217382PurposepTrc99A-mNeonGreen-mcdB for bacterial expressionDepositorInsertmcdB
TagsNeonGreenExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
4QQD
Plasmid#61947PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1:SDC130-H02:C39674 (UHRF1 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPBO.ACT003
Plasmid#182713PurposeaTc inducible dCasRx-IF3DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 3ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ETh_rhi483
Plasmid#180429PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ETh_rhi483
ExpressionBacterialAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP1
Plasmid#67862Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)DepositorInsertMRPP1 (TRMT10C Human)
TagsHisExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-tdTomato-EC18158
Plasmid#137078PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInserttdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only