32,963 results
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Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialPromoterPotefAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mClover LIC cloning vector (MBP-mClover)
Plasmid#86930PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mCloverDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7Available SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (H6-mClover)
Plasmid#86931PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mCloverDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
ExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchG WT
Plasmid#87107Purposeexpresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosaDepositorInsertpchG WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA3
Plasmid#80747PurposeExpression of human beta A3-crystallin in E. coliDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLacIq-Wasabi
Plasmid#86112PurposemWasabi reporter gene driven by LacIq promoterDepositorInsertmWasabi
Tags6xHisExpressionBacterialPromoterpLacIqAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-51
Plasmid#79966PurposeLate1-O3-33-myc-PHDepositorInsertEPN-51
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-11
Plasmid#79926PurposePalm1-Late1-I3-01-mycDepositorInsertEPN-11
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQhsCDtRNA-
Plasmid#86015PurposeMulti-gene expression system with human box C/D proteins with U14DepositorInsertsUseCo-expression; purificationTags6x-HisExpressionBacterialMutationamino acids 1-401, amino acids 1-411, and amino a…Available SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII
Plasmid#80860PurposePhagemid (encodes evolved cI5C6A,P and M13 gene III)DepositorInsertsM13 gene III
cI5C6A,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CHK2_HUMAN_D0
Plasmid#79715PurposeThis plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK1_HUMAN_D0
Plasmid#79725PurposeThis plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK3_HUMAN_D0
Plasmid#79689PurposeThis plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only