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p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p348_DG

Plasmid

#234678

Purpose

Golden gate domesticated pET28b with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p350_DG

Plasmid

#234680

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p345_DG

Plasmid

#234675

Purpose

Golden gate domesticated pET28b with His10-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p355_DG

Plasmid

#234698

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p352_DG

Plasmid

#234682

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p363_DG

Plasmid

#234685

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Sortase

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-bdNEDD8 and Sortase

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pISFba1reRNA

Plasmid

#226822

Purpose

Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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E. coli expression: Z-lock cofilin dark state mutant

Plasmid

#141135

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Bacterial

Mutation

D3 deletion, I32F in Zdk2

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1D1_Hormone-recep

Plasmid

#109960

Purpose

Protein expression and purification of NR1D1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ETS2_pet28a

Plasmid

#131664

Purpose

Bacterial expression of ETS gene ETS2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETS2 (ETS2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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GPX3

Plasmid

#38899

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GPX3 (GPX3 Human)

Tags

Tev His Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pJUMP46-2A(sfGFP)

Plasmid

#126990

Purpose

Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag hDPY30

Plasmid

#15556

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
UBC13-PGEX4T

Plasmid

#25464

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

Ubc13 (UBC13, MMS2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-TTGZFP-FokI-DD

Plasmid

#18755

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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