30,810 results

1. pET15bLCFPT7

   Plasmid

   #53545

   Purpose

   Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

   Depositor

   Lingchong You

   Article

   Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

   Insert

   T7 RNAP
   

   Expression

   Bacterial

   Available Since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+) 6xHis-TEV-IFIT3

   Plasmid

   #53559

   Purpose

   bacterial expression of IFIT3

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT3 (IFIT3 Human)
   

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

3. USP21 (3MTN)

   Plasmid

   #28112

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP21 (USP21 Human)
   

   Tags

   His tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   catalytic domain only (amino acid residues 209-56…

   Available Since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS1: pHelper(AvCAST)_AvPSP1

   Plasmid

   #168133

   Purpose

   Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry-LOV2 I427V

   Plasmid

   #81044

   Purpose

   mammalian expression of LOV2 I427V

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV I427V
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   I427V

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-Nedd-4/2522

   Plasmid

   #11466

   Depositor

   Allan Weissman

   Article

   Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 52-777

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pRS-45

   Plasmid

   #40586

   Depositor

   Harald Schwalbe

   Article

   Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

   Insert

   yTAND12-TEV-hTBPc (TAF1, TBP Budding Yeast, Human)
   

   Tags

   His-tag and yTAND12

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. MP-P6

   Plasmid

   #69665

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR dinB
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT5T_hp53_ST

    Plasmid

    #34922

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 1-393) (TP53 Human)
    

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLBH546_Tet-Cas14b1Locus

    Plasmid

    #112503

    Purpose

    Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. As2

    Plasmid

    #123160

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR-ABS84, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGR-BBa_B0010

    Plasmid

    #46003

    Depositor

    Christopher Voigt

    Article

    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

    Insert

    BBa_B0010 Terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEmpty

    Plasmid

    #214051

    Purpose

    Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pflaG

    Plasmid

    #45477

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Expression

    Bacterial

    Promoter

    flaA

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pSpal2At

    Plasmid

    #78286

    Purpose

    Expresses A. thaliana PAL2 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

    Insert

    PAL2 (PAL2 Mustard Weed)
    

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    lac

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hnRNPA2_266-341

    Plasmid

    #118821

    Purpose

    Bacterial expression of hnRNPA2 truncation

    Depositor

    Nicolas Fawzi

    Article

    Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 266-341

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFTK020

    Plasmid

    #171292

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PalcA syn_NoCrea (ANIA_08979) promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_TNK2-1/1

    Plasmid

    #91377

    Purpose

    Protein expression and purification of human SH3 domain construct TNK2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK2-1/1 (TNK2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRCP

    Plasmid

    #195862

    Purpose

    Encodes chromogenic protein (CP). CP inducible with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Chromogenic Protein
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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