32,967 results
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Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV SPOP 1-374
Plasmid#197015PurposeE. coli expression vectorDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
TagsHis tag, FLAG tagExpressionBacterialPromoterT7Available SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-Pnf
Plasmid#198321PurposeFor affinity purification of Pnf payloadDepositorInsertTwinStrep-SUMO-Pnf
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-ALFA
Plasmid#198301PurposeProduces PVCs retargeted with ALFA tagDepositorInsertPVCpnf_pvc13-ALFA
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-MoonTag
Plasmid#198299PurposeProduces PVCs retargeted with MoonTagDepositorInsertPVCpnf_pvc13-MoonTag
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-SENP2
Plasmid#196355Purposeprotein expression of the His-tagged catalytic domainDepositorInsertSENP2 catalytic domain
TagsHis6-TEVExpressionBacterialMutationcontains only D364-L589PromoterT7Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
TagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorArticleInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV WT SPOP 28-359
Plasmid#194115PurposeWild type His-SUMO SPOP expression plasmidDepositorAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTW160C
Plasmid#194801PurposePCadBA3 expressing LuxABDepositorInsertLuxAB
ExpressionBacterialAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-Cbx5
Plasmid#194547PurposeBacterial Expression of 6xHis mCherry-HP1a Fusion ProteinDepositorInsertCbx9 (HP1a)
TagsmCherryExpressionBacterialAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P*-R
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP* (modified T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R*
Plasmid#189852PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R*-knt
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP (T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only