32,457 results
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Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIRT6
Plasmid#41564PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
TagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD-0016
Plasmid#12345DepositorAvailable SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
ExpressionBacterialMutationD10A, H840APromoterpBADAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)
Plasmid#166421PurposeExpress pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)DepositorInsertNaegleria fowleri PRL
TagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K38A)-StrepII
Plasmid#174423PurposeExpresses human Drp1 Isoform 3 with K38A mutation in bacteriaDepositorInsertDrp1
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK38APromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCLS1-1/1
Plasmid#91437PurposeProtein expression and purification of human SH3 domain construct HCLS1-1/1DepositorInsertHCLS1-1/1 (HCLS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-β′-COPI-WD40
Plasmid#180753PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).DepositorInsertβ′-COPI-WD40 (SEC27 Budding Yeast)
TagsN-terminal strep tag, His-tag, and SUMO tagExpressionBacterialMutationEncodes residues 1-301; deleted 302-889; mutated …PromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATAD2
Plasmid#38916PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR-BluntII-TOPO-RhoC
Plasmid#14099DepositorInsertHuman RhoC (RHOC Human)
ExpressionBacterialAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originExpressionBacterialAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
TagsHaloTagExpressionBacterialPromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-NOR
Plasmid#62306Purposesynthetic circuit: NOR gateDepositorInsertsA2NT sgRNA
A2NT sgRNA
UseCRISPRExpressionBacterialPromoterPPhlF and pBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
L1-1180
Plasmid#13265DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAB094e
Plasmid#79245PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (Y87A); PC araC
ExpressionBacterialPromoterPBAD; PCAvailable SinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only