33,532 results

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pRMC2-tat:msfGFP

Plasmid

#194915

Purpose

Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

Depositor

Rikke Meyer

Publication

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-88)-Strep

Plasmid

#196214

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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S100A6

Plasmid

#196217

Purpose

Codon-optimized bacterial expression plasmid. Expresses S100A6.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

S100A6 (S100A6 Human)

Expression

Bacterial

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pFF-41.5-mCherry

Plasmid

#192863

Purpose

pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

Depositor

Morten Sommer

Publication

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Oxygen inducible promoter

Expression

Bacterial

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197662

Purpose

Expresses GFP under the control of J23100 and RiboJ-UTR1

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE

Plasmid

#188946

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVS_AF

Plasmid

#188950

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AF scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVC_AG

Plasmid

#188954

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AG scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.1

Plasmid

#193802

Purpose

Protein production of ChemoG3.1 in bacteria

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.1

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K, T225R

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG5

Plasmid

#193805

Purpose

Protein production of ChemoG5 in bacteria

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Dhh1

Plasmid

#201412

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Publication

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget-RFP-GFP operon

Plasmid

#192279

Purpose

RFP GFP fusion protein expressed under a constitutive promoter

Depositor

John van der Oost

Publication

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP GFP

Expression

Bacterial

Promoter

PlacIq

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOPARA1

Plasmid

#200416

Purpose

Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)

Depositor

Kamil Witek

Publication

Milnes et al bioRxiv 2023.05.31.543011

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

Plasmid

#194210

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

Depositor

Chase Beisel

Publication

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and CJ8421_04975 Rptr2

Expression

Bacterial

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDS170-enNTS1

Plasmid

#199152

Purpose

JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

Depositor

Daniel Scott

Publication

Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

Insert

enNTS1 (Ntsr1 Rat)

Tags

10xHis tag, Avi tag, MBP, and muGFP

Expression

Bacterial

Mutation

V208M, C273S, C391S and C393S (over NTS1-B5)

Promoter

Lac/T5

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Publication

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Expression

Bacterial

Promoter

Lac promoter and T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Publication

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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