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pHAT4

Plasmid

#112585

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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p045

Plasmid

#70057

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with pDC184

Depositor

Axel Brunger

Article

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

C2AB-linker-SNAP-25_C
SNAP-25_N

Tags

No tags

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MP-K12

Plasmid

#69656

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA dinB

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSA3

Plasmid

#52263

Purpose

Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPK

Plasmid

#38334

Depositor

Florian Freimoser

Insert

E. coli polyphosphate kinase (ppk Escherichia coli)

Tags

6xHIS, MBP, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBR322 14-3-3 Promoter

Plasmid

#16517

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 promoter (SFN Human)

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pMSP1D1G1

Plasmid

#97325

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1G1

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of G residue after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBbA5a-RFP

Plasmid

#35280

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pJ2076.2_cL

Plasmid

#140668

Purpose

cL control for toggle switch experiment

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

cL control for toggle switch experiment

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pGL001

Plasmid

#115674

Purpose

Fluorescent reporter for E.coli K12 fliC promoter activity

Depositor

Gene-Wei Li

Article

Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

Insert

fliC promoter fused with GFP

Tags

Green fluorescent protein (GFP)

Expression

Bacterial

Promoter

fliC promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S14

Plasmid

#128596

Purpose

Expresses tagless 30S ribosomal subunit protein S14 in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S14 (rpsN E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pZE27GFP5

Plasmid

#75458

Purpose

Plasmid for constitutive expression of gfp-pdt5

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#5

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO PuroR

Plasmid

#68447

Purpose

TOPO construct expressing PuroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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PaBphP 1-497

Plasmid

#36920

Depositor

Keith Moffat

Article

Yang et al Nature. 2011 Oct 16;479(7373):428-32. do

Insert

PaBphP-PCD

Tags

His

Expression

Bacterial

Mutation

Contains the photosensory core domain coding regi…

Promoter

T7

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
K401-Micro

Plasmid

#122485

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

His tag MBP Kinesin 1-401 SspB micro

Tags

H6-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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