33,539 results

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Publication

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Expression

Bacterial

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT195

Plasmid

#202294

Purpose

Type IIS cloning plasmid for LPattB7 integrations w/ OriT

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT248

Plasmid

#202289

Purpose

RPU integration plasmid (LPattB2)

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-mNeonGreen

Plasmid

#205017

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-M(BRCT)TdTwt

Plasmid

#207459

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

Depositor

Jay Keasling

Publication

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

M(BRCT)TdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNZ18-CAH

Plasmid

#203159

Purpose

Replicates in E. coli, S. cerevisiae, and A. laidlawii

Depositor

Bogumil Karas

Publication

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-native

Plasmid

#200001

Purpose

Violacein pathway. Native promoter and CDS sequence

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Publication

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Publication

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGS

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-552

Plasmid

#186017

Purpose

2xHis-LRRK2 Armadillo 1-552

Depositor

Suzanne Pfeffer

Publication

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)

Expression

Bacterial

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab1a

Plasmid

#203687

Purpose

Expresses N8C_Fab1a

Depositor

Brenda Schulman

Publication

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab1a

Expression

Bacterial

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pV1F

Plasmid

#205084

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Publication

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Expression

Bacterial

Promoter

agorbacterium virB operon promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

Plasmid

#202023

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

Depositor

Mikhail Shapiro

Publication

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)

Expression

Bacterial

Mutation

GvpA T6A-I48V

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNIC28-BIRC7

Plasmid

#196363

Purpose

protein expression of the His-tagged full length protein

Depositor

Stefan Knapp

Insert

BIRC7/Livin

Tags

His6-TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKM263-USP7

Plasmid

#196365

Purpose

protein expression of the GST-tagged catalytic domain

Depositor

Stefan Knapp

Insert

USP7 catalytic domain

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

contains only S207-E560

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pIA034

Plasmid

#200421

Purpose

amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Publication

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsdronPA

Expression

Bacterial

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RS35075_pMQ30_KO_Kan

Plasmid

#185390

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

Depositor

Adam Arkin

Publication

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker

Expression

Bacterial

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_TypeIC_Cascade_cas3_invivo

Plasmid

#196400

Purpose

expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

Depositor

David Taylor

Publication

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Inserts

Cas5c
Cas7c
Cas8c
Cas3c

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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