8,086 results

1. pFA6a-link-yomKeima-SpHis5

   Plasmid

   #44847

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKeima
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRF1-GW mNeongreen-T2A-mCherry

   Plasmid

   #125698

   Purpose

   Produces equimolar expression of mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0758

   Plasmid

   #133511

   Purpose

   Expression of 3GFP tagged RasAct probe to monitor active Ras1

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV0783

   Plasmid

   #133515

   Purpose

   Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT11F

   Plasmid

   #165062

   Purpose

   Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXP222

   Plasmid

   #26833

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTU-DO-LEU-C4

   Plasmid

   #160341

   Purpose

   High-copy expression vector for K. marxianus, leucine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJZC504

   Plasmid

   #62280

   Purpose

   PCP-VP64 fusion

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   PCP-VP64
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Promoter

   pAdh

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available Since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. BS-His3

    Plasmid

    #69197

    Purpose

    complementation of yeast auxotrophic marker HIS3

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    HIS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Use

    Tags

    FRB and GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL_yEGFP_hc

    Plasmid

    #98746

    Purpose

    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Insert

    Yeast-enhanced green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    synthetic

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV0663

    Plasmid

    #133478

    Purpose

    Expression in fission yeast from promoter pact1, integrated at his5 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p405CYC1

    Plasmid

    #15967

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    p405CYC1 (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRFLIP42

    Plasmid

    #65518

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-mCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRFLIP35

    Plasmid

    #65513

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDRFLIP32

    Plasmid

    #65511

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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