8,086 results

1. YIplac211-GGA2-msGFPx3

   Plasmid

   #105258

   Purpose

   Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Gga2 portion (GGA2 Budding Yeast)
   

   Use

   Tags

   msGFPx3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-LEU-PmCDA1

   Plasmid

   #60818

   Purpose

   to overexpress lamprey deaminase in yeast

   Depositor

   Youri Pavlov

   Article

   Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

   Insert

   CDA1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGK415-­luxTA2-­4F

   Plasmid

   #165971

   Purpose

   Expresses luxTA(2-­4F) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   luxR
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   S116Y, W201R, codon-optimized for expression in y…

   Promoter

   pPGK1 mutant

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA CRU HISMX

   Plasmid

   #131164

   Purpose

   PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA NuiHA-loxP-nat-CUP1p

   Plasmid

   #131170

   Purpose

   PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   Nui-HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179067

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-ERG6-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

   Plasmid

   #179066

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-ATG8
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMC-XII5

   Plasmid

   #176161

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADH1pro_GCN4_SIR4

   Plasmid

   #138620

   Purpose

   Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

   Depositor

   Rob Mitra

   Insert

   GCN4 (GCN4 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179060

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PIL1-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PhyB-mCherry-Htb2

    Plasmid

    #51569

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    HTB2 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-G11-5FLAG-kanMX6

    Plasmid

    #49182

    Purpose

    genomic G11-5FLAG epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    G11-5FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYES2.1-cerMET16

    Plasmid

    #107442

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET16 (MET16 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. YIplac211-ANP1-msGFP2

    Plasmid

    #177100

    Purpose

    Expresses Anp1-msGFP2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ANP1 (ANP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p415PGK1-AtMAX1(pYL759)

    Plasmid

    #178289

    Purpose

    Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    AtMAX1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-N2H-F1-empty vector

    Plasmid

    #125551

    Purpose

    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVG49

    Plasmid

    #164707

    Purpose

    yEVenus under the control of GAL1 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    GAL1pr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG303GAL-ccdB-EGFP

    Plasmid

    #14181

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGPD-luxAB(HIS)

    Plasmid

    #1106

    Depositor

    Susan Lindquist

    Article

    Parsell et al Nature 1994 Dec 1;372(6505):475-8.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only