8,060 results

1. pMC-Ura-Cen-lvl1

   Plasmid

   #176182

   Purpose

   preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMC-X2

   Plasmid

   #176152

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC-X3

   Plasmid

   #176153

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC-XI1

   Plasmid

   #176155

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMC-XI5

   Plasmid

   #176158

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC-XII2

   Plasmid

   #176160

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV0523

   Plasmid

   #133495

   Purpose

   Expression of nucleo-cytoplasmic sfGFP in M-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(mam1*)-sfGFP-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJW1665

   Plasmid

   #112039

   Purpose

   Integrating plasmid for expression of ER localized mCherry at TRP1 locus

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   ER-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR-GW FLIPglu-2µΔ13V

   Plasmid

   #28000

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-2µΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   H152A

   Promoter

   Available since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pBM5218

    Plasmid

    #18796

    Depositor

    Mark Johnston

    Article

    Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

    Insert

    Ty5-HIS3AI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pRSCupGFP

    Plasmid

    #1086

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pTSK450

    Plasmid

    #34552

    Depositor

    James McNally

    Insert

    MS2-CP-NLS
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG424GPD-ccdB-HA

    Plasmid

    #14248

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 423Gal-FUS-YFP

    Plasmid

    #29615

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pFA6a-G9-5FLAG-kanMX6

    Plasmid

    #49181

    Purpose

    genomic G9-5FLAG epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    G9-5FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Vimentin/pAS2-1

    Plasmid

    #98136

    Purpose

    Vimentin (NM_003380) in DNA-binding domain (BD) vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pXP420 v2 (repaired Amp promoter)

    Plasmid

    #86920

    Purpose

    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.

    Depositor

    Gary Yellen

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPTK018-3b-RFP_Sec

    Plasmid

    #84977

    Purpose

    Type 3b, Red fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1677 c to g and 1794 a to g)

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge