8,086 results

1. pRG223MX

   Plasmid

   #64523

   Purpose

   Episomal E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG414GPD-GaCYP722C(pYL777)

   Plasmid

   #178290

   Purpose

   Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   GaCYP722C
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAH6

   Plasmid

   #41830

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7,TEF

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3039(XII-2 MarkerFree)

   Plasmid

   #73279

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Vimentin/pAS2-1

   Plasmid

   #98136

   Purpose

   Vimentin (NM_003380) in DNA-binding domain (BD) vector, used for yeast-two hybrid

   Depositor

   Rita Shiang

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAV0770

   Plasmid

   #133518

   Purpose

   Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG424GAL-ccdB-HA

   Plasmid

   #14247

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF1-GW Electra1-T2A-mCherry

   Plasmid

   #205498

   Purpose

   Expression of Electra1-T2A-mCherry in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   Electra1-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAT-TET-OFF -LEU- 4xFLAG

   Plasmid

   #182527

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Use

   Tags

   4XFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

    Plasmid

    #179035

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-SCS2tmh
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Spt2-GFP

    Plasmid

    #115572

    Purpose

    Expresses yeast Spt2-GFP fusion protein

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    SPT2
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p41Nat MagicFluor

    Plasmid

    #58563

    Purpose

    p41Nat carrying both mating type markers buffered by the Cyc1 terminator

    Depositor

    Leonid Kruglyak

    Insert

    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYEXC3GH

    Plasmid

    #49027

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGM-ENO2-mCherry-YFP

    Plasmid

    #180570

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDB1751

    Plasmid

    #174013

    Purpose

    pDUAL plasmid expressing GFP from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    GFP
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1 and 41nmt1 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRF1-GW eGFP-T2A-mCherry

    Plasmid

    #118422

    Purpose

    Produces equimolar expression of eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    eGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV0612

    Plasmid

    #133505

    Purpose

    Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG305GAL-EGFP-ccdB

    Plasmid

    #14305

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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