8,060 results

1. pCfB6684

   Plasmid

   #106154

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6631

   Plasmid

   #106161

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXRA2

   Plasmid

   #63144

   Purpose

   ADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2374

   Plasmid

   #67534

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNRB143

   Plasmid

   #36452

   Depositor

   Douglas Bishop

   Article

   Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

   Insert

   RAD54 (RAD54 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

6. p415 Gcs1

   Plasmid

   #112646

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Gcs1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHES873

   Plasmid

   #87945

   Purpose

   Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2875

   Plasmid

   #63640

   Purpose

   pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-zeoCV

    Plasmid

    #73890

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    zeoCV
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Jeffrey Caplan, Nicole Donofrio

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Rp27

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

12. cyterm-ymScarletI pDRF1-GW

    Plasmid

    #168082

    Purpose

    Expresses Cyterm-ymScarletI to check for monomericity.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    cyterm-ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW mKate2-T2A-mTurquoise2

    Plasmid

    #118426

    Purpose

    Produces equimolar expression of mKate2 and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mKate2-T2A-mTq2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HsSPHK2

    Plasmid

    #118092

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS415-recR

    Plasmid

    #49457

    Purpose

    single copy recombinase yeast expression plasmid

    Depositor

    Marc Gartenberg

    Article

    Ansari et al Methods. 1999 Feb;17(2):104-11.

    Insert

    recR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDRF1-GW AKAR3-EV-NR

    Plasmid

    #182534

    Purpose

    Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

    Insert

    AKAR3-EV-NR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T506A mutation

    Promoter

    PMA1

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS1837

    Plasmid

    #123058

    Purpose

    _-factor pheromone

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    _-factor [3-4a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pESC-NAT-Ago-Dcr

    Plasmid

    #160381

    Purpose

    Overexpression of Ago-Dcr fusion protein in yeast

    Depositor

    John McCusker

    Insert

    Ago- Dcr
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS10

    Plasmid

    #83795

    Purpose

    Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGT572_Nemo

    Plasmid

    #153487

    Purpose

    Level 1 episomal expression plasmid for S. cerevisiae

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge