8,060 results

1. YIplac204-pOst1-ESCargo*

   Plasmid

   #115427

   Purpose

   Expresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p404GALS

   Plasmid

   #17435

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALS promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046692

   Plasmid

   #149195

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRS314-hUD

   Plasmid

   #11005

   Depositor

   Francisco Malagon

   Insert

   hUD recombination system
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Direct repeat recombination system. Recombination…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416GPD-ccdB-EYFP

   Plasmid

   #14220

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

   Plasmid

   #89071

   Purpose

   genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

   Depositor

   Quanwen Jin

   Article

   Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

   Type

   Empty backbone

   Use

   Genomic integration in s. pombe

   Tags

   GBP-mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoofy42

   Plasmid

   #55190

   Purpose

   Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXRL2

   Plasmid

   #63148

   Purpose

   Leu2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPS293

   Plasmid

   #8851

   Depositor

   Pamela Silver

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pSK260

    Plasmid

    #129252

    Purpose

    dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35 NM yTRAP sensor reporting on mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSD02

    Plasmid

    #16009

    Depositor

    Fred Cross

    Article

    Di Talia et al Nature. 2007 Aug 23. 448(7156):947-51.

    Insert

    ACT1pr-DsRed.T1.N1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICE ADE2-HphMx

    Plasmid

    #122895

    Purpose

    ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYES2.1-cerMET2

    Plasmid

    #107435

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET2 (MET2 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PhyB-mCherry-SNF7

    Plasmid

    #51574

    Purpose

    Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    SNF7 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYES2.1-cerMET3

    Plasmid

    #107436

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET3 (MET3 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYES2.1-cerMET17

    Plasmid

    #107443

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET17 (MET17 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-mKO1-CaUra3

    Plasmid

    #44638

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge