8,086 results

1. LHP462 - Ub-HA

   Plasmid

   #32169

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pFA6a-link-yoPS-CFP2-Kan

   Plasmid

   #44948

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETcon(I-LtrI)

   Plasmid

   #41523

   Depositor

   Andrew Scharenberg

   Insert

   I-LtrI
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YCPlac33-sGFP-VRG4

   Plasmid

   #25447

   Depositor

   Benjamin Glick

   Article

   Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

   Insert

   Synthetic Green Fluorescent Protein - VRG4
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. BiFC3

   Plasmid

   #190048

   Purpose

   BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (ATG-aa173-238)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. BiFC4

   Plasmid

   #190049

   Purpose

   BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (ATG-aa173-238)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BiFC2

   Plasmid

   #190047

   Purpose

   BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (aa1-172 - TAA)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BiFC1

   Plasmid

   #190046

   Purpose

   BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Insert

   YemVenus (aa1-172)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met3

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-link-yoEmerald-Kan

   Plasmid

   #44946

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEmerald
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMEG_BB3_YL001

    Plasmid

    #117825

    Purpose

    Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_YlGUT1_YlCyc1TT
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-bleMX6-Purg1-3FLAG

    Plasmid

    #19357

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72230

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJZC625

    Plasmid

    #62321

    Purpose

    sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA +1x MS2, Pol II promoter with ribozyme cleavage
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GTH-y

    Plasmid

    #81105

    Purpose

    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-hphMX6-P41nmt1-3FLAG

    Plasmid

    #19349

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. YIplac211-CHC1-msGFPx3

    Plasmid

    #105256

    Purpose

    Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Chc1 portion (CHC1 Budding Yeast)
    

    Use

    Tags

    msGFPx3

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB8383

    Plasmid

    #126917

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    tHMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge