8,432 results
-
Plasmid#168082PurposeExpresses Cyterm-ymScarletI to check for monomericity.DepositorInsertcyterm-ymScarletI
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pAW8-aid-5Myc-Turg1:kanMX6
Plasmid#169356PurposeC-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombeDepositorInsertauxin-inducible degron (aid)
Tags5XMycExpressionYeastAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-
-
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterMET17Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITI1
Plasmid#166094PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-RFP
Plasmid#166841PurposeExpresses Vac8-RFP in yeastsDepositorAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRR2
Plasmid#166097PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP403red-hENT1
Plasmid#166027PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpTDH3Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-ptetO7-crtYBI
Plasmid#165975PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC66A, C1359T and C1281A, G1698APromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-rtetTAK8N, L131L
Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG45
Plasmid#164703PurposeyEVenus under the control of CUP1 promoterDepositorInsertCUP1pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG48
Plasmid#164706PurposertTA under the control of PGK1 promoterDepositorInsertPGK1pr - rtTA - CYC1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-HphMx
Plasmid#122898PurposeLYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-KanMx
Plasmid#122902PurposeTRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only