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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK020

Plasmid

#171292

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PalcA syn_NoCrea (ANIA_08979) promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEDJ333

Plasmid

#177272

Purpose

GAL1p:Cas9:CYC1t

Depositor

Michael Jensen

Publication

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

Cas9

Expression

Bacterial and Yeast

Promoter

GAL1

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK010

Plasmid

#171282

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PxlnA ANIA_03613 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SYS1-msGFPx3

Plasmid

#177112

Purpose

Expresses Sys1-msGFP3 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SYS1 (SYS1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_1_dCas9_sgRNA

Plasmid

#163710

Purpose

Yeast low copy plasmid with dCas9 and sgRNA expression cassette

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL498

Plasmid

#169154

Purpose

Encodes OptoQ-INVRT4

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL724

Plasmid

#169151

Purpose

Encodes OptoQ-AMP5

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-URA3

Plasmid

#122907

Purpose

ADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-URA3

Plasmid

#122886

Purpose

ADE2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MmSphk2

Plasmid

#118598

Purpose

Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

Depositor

Kevin Lynch

Publication

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pYeDP60-hATP13A2-E343A

Plasmid

#171378

Purpose

A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

Depositor

Peter Vangheluwe

Publication

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 variant 2 wild-type (ATP13A2 Human)

Tags

BAD tag

Expression

Yeast

Mutation

E343A

Promoter

URA3

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pV1393:SpRY

Plasmid

#172189

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Publication

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Publication

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHES262 [prADH1-Msn2-mCherry, pnh604]

Plasmid

#103844

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Publication

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pAdh1-Msn2-mCherry

Tags

mCherry

Expression

Yeast

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-URA3

Plasmid

#122915

Purpose

TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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ADH1pro_GCN4_SIR4

Plasmid

#138620

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Insert

GCN4 (GCN4 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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