8,086 results
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Plasmid#32440DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only
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pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationERG20 N127WPromoterAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
UseTagsExpressionYeastMutationPromoterLEU1prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker=deltakPromoterAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXP122
Plasmid#26827DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAV0586
Plasmid#133473PurposeIntegration into fission yeast genome at lys3 locus with bsdMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable SinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM2-mRUBY2
Plasmid#107793Purposedonor vector for knock-in gene editing at human DNM2 locusDepositorInsertDNM2-mRuby2 (DNM2 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)
Plasmid#178995PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0211
Plasmid#8734DepositorInsertyECitrine A206R
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206RPromoterAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1
Plasmid#18684DepositorAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only