8,173 results

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YIplac211-STV1-iGFP

Plasmid

#177111

Purpose

Expresses Stv1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STV1 (STV1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAH1

Plasmid

#41825

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

Depositor

Melissa Moore

Article

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Bacterial and Yeast

Promoter

T7, TEF

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FRP1432

Plasmid

#64509

Purpose

Expresses destabilized Citrine in yeast.

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Citrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-S-tag-hphMX4

Plasmid

#20767

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFS118

Plasmid

#12378

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

ura4, thymidine kinase

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK369

Plasmid

#73594

Purpose

Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

Depositor

T. J. Kappock

Insert

delta-12 fatty acid acetylenase

Tags

FLAG tag

Expression

Yeast

Promoter

GAL10

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pXP314

Plasmid

#26835

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pHpgRNA50

Plasmid

#160128

Purpose

To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Article

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-LSC2- HDV-TAMO

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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POM42-mCitrine

Plasmid

#69854

Purpose

N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPS2167

Plasmid

#1365

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pBTM116-D9

Plasmid

#111232

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Article

Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

Insert

ccdB-cassette

Expression

Yeast

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-MYO1

Plasmid

#51570

Purpose

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

MYO1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pIRTetR-GAL80

Plasmid

#185853

Purpose

Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179077

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-weakSC_GFP

Plasmid

#22315

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

GFP-rad9

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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