8,223 results
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Plasmid#14298DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only
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pGBW-m4046577
Plasmid#145395PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mVenus-T2A-mTurquoise2
Plasmid#118434PurposeProduces equimolar expression of mVenus and mTq2DepositorInsertmVenus-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:mTREK-1_cryst
Plasmid#133269PurposeP. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFPDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM2-mRUBY2
Plasmid#107793Purposedonor vector for knock-in gene editing at human DNM2 locusDepositorInsertDNM2-mRuby2 (DNM2 Synthetic, Human)
UseYeast-e.coli shuttle vectorExpressionBacterial and YeastAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorExpressionBacterial and YeastAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)
Plasmid#178995PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179033PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Neon(gfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTn
Plasmid#154369PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPTK017-3b-yEGFP_Sec
Plasmid#84976PurposeType 3b, Green fluorescent proteinDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIEforRed
Plasmid#185892PurposeIn vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locusDepositorInsertPRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046327
Plasmid#153913PurposeYeast expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpTEF1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPTK013-3a-Invertase-αMFΔ
Plasmid#84972PurposeType 3a, Invertase followed by αMFΔ - secretion signalDepositorInsertInvertase followed by αMFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLUM
Plasmid#64169Purposeharbours marker genes LEU2, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP522
Plasmid#26847DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB-DsRed
Plasmid#14367DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 153-405-YFP
Plasmid#18689DepositorInsertRnq1 153-405
TagsYFPExpressionYeastMutationPrion domain of Rnq1Available SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKT0101
Plasmid#8721DepositorInsertyECFP
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163AAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only