8,430 results

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TM104b(WT)

Plasmid

#1159

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Publication

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBK1

Plasmid

#1213

Depositor

Susan Lindquist

Publication

Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

Insert

Hsc82::LEU2 (HSC82 Budding Yeast)

Use

Integration

Expression

Yeast

Mutation

hsc82::LEU2 deletion select for plasmid using LE…

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(WT)

Plasmid

#1162

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(WT)

Plasmid

#1165

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

K351N

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-URA3

Plasmid

#122911

Purpose

LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 31, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-NLS-tdSG

Plasmid

#255013

Purpose

expression of MCP-NLS-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Plon1-MCP-NLS-tdSG

Plasmid

#255010

Purpose

expression of MCP-NLS-tandemStayGold from weak P.lon1 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.lon1(451-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.lon1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-NLS-tdSG

Plasmid

#255011

Purpose

expression of MCP-NLS-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Ppak1-MCP-NLS-tdSG

Plasmid

#255012

Purpose

expression of MCP-NLS-tandemStayGold from weak P.pak1 (P.shk1) promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.pak1(630-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.pak1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-2xNLS-NES-tdSG

Plasmid

#255014

Purpose

expression of MCP-2xNLS-NES-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-2xNLS-2xNES-tdSG

Plasmid

#255015

Purpose

expression of MCP-2xNLS-2xNES-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-2xNLS-NES-tdSG

Plasmid

#255016

Purpose

expression of MCP-2xNLS-NES-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG

Plasmid

#255017

Purpose

expression of MCP-2xNLS-2xNES-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-stdMCP-NLS-tdSG

Plasmid

#255018

Purpose

expression of tandemMCP-NLS-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-stdMCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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