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pMTKM05

Plasmid

#233477

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Publication

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKS1576

Plasmid

#239524

Purpose

Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

Depositor

John Dueber

Insert

ScVba2p-mTurquoise2-2xCrk_SH3

Expression

Yeast

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRO227

Plasmid

#233835

Purpose

MoClo-YTK; ConL1/RE cassette; rtTA systems; tetO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

tetO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO236

Plasmid

#233847

Purpose

MoClo-YTK; ConL2/RE cassette; ABA systems; phlfO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN020

Plasmid

#233850

Purpose

MoClo-YTK; ConL2/RE cassette; Auxin systems; amtRO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN069

Plasmid

#233855

Purpose

MoClo-YTK; ConL2/RE cassette; NS3 systems; lexAO6-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

lexAO6-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO174

Plasmid

#233809

Purpose

MoClo-YTK; type3A; ABA systems; Phlf-NES

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Phlf-NES

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Nat

Plasmid

#236494

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-G418

Plasmid

#236481

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Publication

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-5

Plasmid

#233532

Purpose

Expresses AvrSr62-5 in yeast

Depositor

Brande Wulff

Publication

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-5

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-1

Plasmid

#233531

Purpose

Expresses AvrSr62-1 in yeast

Depositor

Brande Wulff

Publication

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-1

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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