8,133 results

1. BS-Lys2

   Plasmid

   #69199

   Purpose

   complementation of yeast auxotrophic marker LYS2

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   LYS2
   

   Expression

   Yeast

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICE ADE2-NatMx

   Plasmid

   #122897

   Purpose

   ADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJAID8Z

   Plasmid

   #165053

   Purpose

   Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJAID6A

   Plasmid

   #165046

   Purpose

   Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSJ125

   Plasmid

   #104344

   Purpose

   Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   MDH2
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4046277

   Plasmid

   #145428

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCH001

   Plasmid

   #47953

   Purpose

   tetO-GFP reporter casette

   Depositor

   Attila Becskei

   Article

   Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

   Insert

   GFP
   

   Use

   Tet inducible expression

   Expression

   Yeast

   Promoter

   T3

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RPL25NLS-GFP-PRA (102)

   Plasmid

   #24041

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Tags

   EGFP and PRA

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pGBW-m4046319

   Plasmid

   #153866

   Purpose

   Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046292

    Plasmid

    #149062

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046249

    Plasmid

    #148991

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRS416 Gal TDP43 G294A

    Plasmid

    #27459

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

13. 721-2µ-staHIS3

    Plasmid

    #40611

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Tags

    HA

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ube2g1

    Plasmid

    #31407

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2G1 Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pIM35

    Plasmid

    #74644

    Purpose

    Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    June 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS313-Sad1-Wt

    Plasmid

    #90308

    Purpose

    yeast expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Tags

    no tag

    Expression

    Yeast

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046230

    Plasmid

    #145454

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. LD229

    Plasmid

    #69192

    Purpose

    Integration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg29-2EGFP
    

    Tags

    2xEGFP

    Expression

    Yeast

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046658

    Plasmid

    #149226

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046682

    Plasmid

    #149197

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus)
    

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge