8,418 results
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Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM019_P2_pRPL2102
Plasmid#216444PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rpl2102
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM015_P2_pADH1
Plasmid#216440PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter adh1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM013_P2_pENO101
Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
Tags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-4V5-APEX2-URA
Plasmid#207056PurposeFor ORF tagging with 4V5-APEX2DepositorInsert4xV5-APEX2
Tags4xV5-APEX2ExpressionYeastAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPGP_scFv
Plasmid#183732PurposeExpresses anti-lysozyme scFv antibodyDepositorInsertAnti-lysozyme scFv
TagsHis6-tagExpressionYeastPromoterGAP promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
Tags3xHAExpressionYeastMutation∆RPromoterpATG8Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-VSV
Plasmid#212752PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD100
Plasmid#212924PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionYeastAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-NUbo-GFP-His6
Plasmid#212790PurposeCopper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeastDepositorInsertNUbo-GFP-His6
UseIntegrative vectorExpressionYeastPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
TagsmClover3ExpressionBacterial and YeastPromoterSpH2A/Bpro (divergent)Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only