8,128 results

1. YIplac211-KEX2-iGFPx6

   Plasmid

   #105264

   Purpose

   Plasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Kex2 portion (KEX2 )
   

   Tags

   iGFPx6

   Expression

   Yeast

   Available Since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVEG17*

   Plasmid

   #111856

   Purpose

   Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

   Depositor

   Nikhil Nair

   Article

   Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

   Insert

   GAL1p-GAL3-Syn4.1-TEFt
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   D68N, V69M, A109V, I271L

   Promoter

   GAL1p

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. RS314

   Plasmid

   #17542

   Depositor

   Bert Vogelstein

   Article

   Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pR5-30

   Plasmid

   #22290

   Depositor

   Louise Prakash

   Article

   Pages et al Genetics. 2008 Sep . 180(1):73-82.

   Insert

   RAD5 (RAD5 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   D681, E682->AA

   Available Since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pR5-19

   Plasmid

   #22288

   Depositor

   Louise Prakash

   Article

   Pages et al Genetics. 2008 Sep . 180(1):73-82.

   Insert

   RAD5 (RAD5 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   C914, C917->AA in the C3HC4 ring-finger motif

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pRG711_lexO-AscI_LexA-TF_HIS3MX

   Plasmid

   #154820

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXRkMX

   Plasmid

   #63147

   Purpose

   Kan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Bacterial and Yeast

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FT1

   Plasmid

   #31435

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Fused Toes Homolog (AKTIP Human)
   

   Tags

   HA

   Expression

   Yeast

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCfB3043(gRNA XI-1)

   Plasmid

   #73285

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDDGFP-Leu2d_GgPCFT

    Plasmid

    #165414

    Purpose

    expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT (SLC46A1 Synthetic, Chicken)
    

    Tags

    GFP-His8

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNC891

    Plasmid

    #20397

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    YFP-reporter
    

    Expression

    Yeast

    Mutation

    X=M (see schematic)

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG416GPD-SEC7-Cerulean

    Plasmid

    #18851

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    SEC7 (SEC7 Budding Yeast)
    

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pKT0232

    Plasmid

    #8727

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP
    

    Tags

    13x Myc and Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. p416TEF-NYFP

    Plasmid

    #99553

    Purpose

    Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

    Depositor

    Liming Li

    Article

    Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

    Insert

    Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
    

    Tags

    YFP

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS788

    Plasmid

    #123059

    Purpose

    _-factor protease

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Bar1 [3]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a- mECitrine-kanMX6

    Plasmid

    #105150

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    mECitrine-kanMX6
    

    Tags

    mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG1-NMGR526

    Plasmid

    #1122

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)
    

    Tags

    GR526

    Expression

    Yeast

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pAS1

    Plasmid

    #12388

    Depositor

    Nick Rhind

    Article

    Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

    Insert

    green fluorescent protein
    

    Expression

    Yeast

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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