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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Publication

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-DHDDS

Plasmid

#203177

Purpose

Centromeric yeast expression vector for hDHDDS

Depositor

William Sessa

Publication

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

RER2

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRS314-prp16-302

Plasmid

#89974

Purpose

prp16-302 allele in TRP plasmid

Depositor

Christine Guthrie

Publication

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-302 allele (R456K and G691R)

Available since

Aug. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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RB793

Plasmid

#199121

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Publication

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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RB794

Plasmid

#199122

Purpose

This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

Depositor

Richard Bennett

Publication

Kakade et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

100 bp barcoding cassette

Expression

Yeast

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-ccdB-6stop

Plasmid

#203511

Purpose

Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-ccdB-6stop

Plasmid

#203514

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-ccdB-6stop

Plasmid

#203512

Purpose

Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-1x-ccdB-6stop

Plasmid

#203519

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-2x-uA-ccdB-6stop

Plasmid

#203517

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-HIV-1_PR

Plasmid

#203493

Purpose

Expresses HIV-1 protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-1 protease

Expression

Yeast

Promoter

GALL

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-WNV_NS2B-GS-NS3

Plasmid

#203475

Purpose

Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

WNV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HRV-B14_3C

Plasmid

#203466

Purpose

Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HRV-B14 3C protease (HRVBgp1 )

Expression

Yeast

Promoter

GAL10

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-HAV_3C

Plasmid

#203481

Purpose

Expresses HAV 3C protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HAV 3C protease

Expression

Yeast

Promoter

GALL

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SARS-CoV-2_PLP

Plasmid

#203509

Purpose

Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-KUNV_NS2B-GS-NS3

Plasmid

#203506

Purpose

Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

KUNV NS2B-GS-NS3

Expression

Yeast

Promoter

GAL1

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPT-TAR C1

Plasmid

#141246

Purpose

Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Publication

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Minor additional mutations described in manuscript

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Publication

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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