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pU-mRuby2

Plasmid

#176549

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of uracil

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL-Sapphire

Plasmid

#176568

Purpose

Expression of Sapphire for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Insert

yETSapphire

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHpgRNA50

Plasmid

#160128

Purpose

To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Article

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-LSC2- HDV-TAMO

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- PGK1prom-TIR1-FLAG

Plasmid

#189724

Purpose

Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Expression

Yeast

Promoter

PGK1 promoter

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Tags

mScarlet fused to FKBP

Expression

Yeast

Promoter

SP6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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HisSumoOphP

Plasmid

#189672

Purpose

HisSUMO tagged OphP to express OphP in pichia pastorias.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

HisSumoOphP

Tags

HisSumo tagged

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-NAT

Plasmid

#188926

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Kan

Plasmid

#188923

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Kan

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Kan

Plasmid

#188918

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Kan

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Phleomycin

Plasmid

#188915

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPAeBlueHPV16LR

Plasmid

#185893

Purpose

2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PALD6>AeBlue>TPGK1- PSe.GAL2> HPV16-L1_C-6*H > TRPL41B

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CaCYP722C(pYL803)

Plasmid

#178291

Purpose

Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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