8,133 results

1. pREP4xN_ccdB

   Plasmid

   #79022

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG413GAL-Cerulean-ccdB

   Plasmid

   #14405

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM01

   Plasmid

   #135414

   Purpose

   Expression of Hygromycin resistance from CMV promoter

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   CMVpr hph ADH1t
   

   Expression

   Bacterial and Yeast

   Promoter

   CMVpr

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPTK015-3-yEGFP_Intra

   Plasmid

   #84974

   Purpose

   Type 3, Green fluorescent protein

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS316-RGR-GFP-mHDV

   Plasmid

   #51058

   Purpose

   Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS316-RGR-GFP-mHH

   Plasmid

   #51057

   Purpose

   Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG415GPD-Cerulean-ccdB

   Plasmid

   #14410

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF’- AmTryoshka1;3-GS

   Plasmid

   #100967

   Purpose

   Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
   

   Expression

   Yeast

   Promoter

   PMA

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWS033

   Plasmid

   #123056

   Purpose

   Green fluorescent protein

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   sfGFP [3]
   

   Expression

   Yeast

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG424GAL-ccdB-EYFP

    Plasmid

    #14223

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P41nmt1-mEGFP

    Plasmid

    #105140

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P41nmt1-mEGFP
    

    Tags

    41nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    41nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LD214

    Plasmid

    #69185

    Purpose

    Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg14-2EGFP
    

    Tags

    2xEGFP

    Expression

    Yeast

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LD205

    Plasmid

    #69180

    Purpose

    Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg5-2EGFP
    

    Tags

    2xEGFP

    Expression

    Yeast

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG424GAL-EYFP-ccdB

    Plasmid

    #14343

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046380

    Plasmid

    #148977

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4046710

    Plasmid

    #149178

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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