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pDIV488

Plasmid

#177696

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSSa31

Plasmid

#179504

Purpose

Tet-on genome insertion system

Depositor

Dominique Sanglard

Publication

Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

Type

Empty backbone

Expression

Yeast

Promoter

Tet-HOP1 (p98CGH)

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSSa32

Plasmid

#179505

Purpose

Tet-on genome insertion system

Depositor

Dominique Sanglard

Publication

Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

Type

Empty backbone

Expression

Yeast

Promoter

Tet-HOP1 (p99CGH)

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pML107-kanR468

Plasmid

#162041

Purpose

Expresses Cas9 and contains kanR468 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Publication

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR468 guide

Use

CRISPR

Expression

Yeast

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-Ura-Cen-lvl1

Plasmid

#176182

Purpose

preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDN513

Plasmid

#175365

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.

Depositor

Daniel Nickerson

Publication

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN523

Plasmid

#175369

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

Depositor

Daniel Nickerson

Publication

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN524

Plasmid

#175370

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

Depositor

Daniel Nickerson

Publication

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN525

Plasmid

#175371

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

Depositor

Daniel Nickerson

Publication

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN526

Plasmid

#175372

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Publication

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-Elo3-GFP11-Ura

Plasmid

#177734

Purpose

ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

Depositor

Erik Böttger

Publication

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

Elo3 (ELO3 Budding Yeast)

Tags

GFP11 and HDEL

Expression

Bacterial and Yeast

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ400

Plasmid

#177269

Purpose

USER cloning-compatible 2μ vector (URA3)

Depositor

Michael Jensen

Publication

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_PCP-VP64_B-PP7

Plasmid

#177157

Purpose

Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

PCP-VP64
B-PP7

Tags

SV40 NLS

Expression

Yeast

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Available since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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