8,128 results

1. 6x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64392

   Purpose

   encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Expression

   Yeast

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAG425GAL-ccdB-ECFP

   Plasmid

   #14177

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTGPD-H-Hsp82

   Plasmid

   #1278

   Depositor

   Susan Lindquist

   Article

   Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

   Insert

   Hsp82 (HSP82 Budding Yeast)
   

   Tags

   Histidinex6

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

   Plasmid

   #126739

   Purpose

   yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2, acs1*, ald6
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, S629N (please see depositor comm…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG423GPD-ccdB-ECFP

   Plasmid

   #14174

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Expression

   Yeast

   Mutation

   See depositor comments below

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB763

   Plasmid

   #60158

   Purpose

   Firefly luciferase (Promega) driven by LYS9 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LYS9pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. BB3aK_14*

   Plasmid

   #98529

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCH043

   Plasmid

   #47949

   Purpose

   rtTA under the control of GAL1 promoter

   Depositor

   Attila Becskei

   Article

   Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

   Insert

   rtTA
   

   Use

   Tet inducible expression

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDH5

    Plasmid

    #83775

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS414 Hsh155 WT

    Plasmid

    #87220

    Purpose

    Encodes Hsh155 WT for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046697

    Plasmid

    #153869

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGBW-m4046702

    Plasmid

    #149158

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGBW-m4046387

    Plasmid

    #149005

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pNC952

    Plasmid

    #20401

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP-reporter
    

    Expression

    Yeast

    Mutation

    X=UbiM (see schematic) linker=deltak

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. S3_004_026_MV1

    Plasmid

    #134416

    Purpose

    pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Expression

    Yeast

    Promoter

    VTC4 (native promoter)

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046447

    Plasmid

    #145387

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

    Plasmid

    #106471

    Purpose

    Expresses Prc1 with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    PRC1
    

    Tags

    PA

    Expression

    Bacterial and Yeast

    Promoter

    PRC1

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

    Plasmid

    #68279

    Purpose

    SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Far1DS NLS mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    RPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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