8,129 results

1. pLU

   Plasmid

   #64174

   Purpose

   harbours marker genes LEU2 and URA3

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-X3

   Plasmid

   #115568

   Purpose

   Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   Green Fluorescent Protein
   

   Expression

   Bacterial and Yeast

   Mutation

   Three repeats of the Gcn5 consensus motif are tag…

   Promoter

   ADH1

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0587

   Plasmid

   #133474

   Purpose

   Integration into fission yeast genome at his5 locus with bleMX selection cassette

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP4010

   Plasmid

   #113336

   Purpose

   Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

   Inserts

   Artificial Transcription Factor (McIsaac et al, 2013)
   Ty3 Integrase
   

   Tags

   3X-FLAG/8X-Gly linker

   Expression

   Yeast

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. piHGpd/p82

   Plasmid

   #1105

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   wild-type HSP82 regulated by the strong constitut…

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pES1002

   Plasmid

   #21789

   Depositor

   Richard Yu

   Article

   Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

   Insert

   Fus3-as2 (FUS3 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   contains 5' 1kb upstream UTR/promoter Q93…

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. 416Gal-FUS-R524T-YFP

   Plasmid

   #29627

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R524T

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pGBW-m4046256

   Plasmid

   #153937

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGBW-m4046627

   Plasmid

   #153916

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 416Gal-FUS-R518K-YFP

    Plasmid

    #29621

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R518K

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. AtAUX1

    Plasmid

    #117087

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtAUX1
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AtPIN1

    Plasmid

    #117088

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtPIN1
    

    Tags

    His

    Expression

    Yeast

    Promoter

    AOX

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG413GPD-ccdB-TAP

    Plasmid

    #14262

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046248

    Plasmid

    #153865

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRS426-ACT_unstable

    Plasmid

    #127608

    Purpose

    high-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    Unstable ACT1 intron (WT)
    

    Expression

    Yeast

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS426-ECM_stable

    Plasmid

    #127606

    Purpose

    high-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    Stable ECM33 intron (WT)
    

    Expression

    Yeast

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDC12(WT)

    Plasmid

    #1162

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Tags

    266bp of upstream sequence

    Expression

    Yeast

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSec7-iGFPx6

    Plasmid

    #115424

    Purpose

    Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-iGFPx6
    

    Tags

    iGFP

    Expression

    Yeast

    Promoter

    Endogenous

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gal ts-lux

    Plasmid

    #1329

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

    Insert

    bacterial luciferase-V.h.MAV
    

    Expression

    Yeast

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGBW-m4046369

    Plasmid

    #149118

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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