8,418 results
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Plasmid#122899PurposeLYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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pICE LYS2-NatMx
Plasmid#122900PurposeLYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HphMx
Plasmid#122901PurposeTRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-SgH
Plasmid#163972PurposeHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGT572_Nemo
Plasmid#153487PurposeLevel 1 episomal expression plasmid for S. cerevisiaeDepositorTypeEmpty backboneExpressionYeastAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
ExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372AAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
ExpressionYeastMutationEnhanced Nucleur Localization SignalAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG425 GAL Pong TPase L418A, L420A
Plasmid#145795PurposemPing Yeast Transposition AssayDepositorInsertPong TPase L418A, L420A
ExpressionYeastMutationL418A, L420AAvailable sinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCTCON2-BXG-2TAG
Plasmid#158138PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HPH
Plasmid#160019PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-LEU
Plasmid#160017PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG830
Plasmid#158601PurposetNCS-ePTS1 (2micron)DepositorInsertpTDH3-tNCS-ePTS1
TagsePTS1ExpressionYeastAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG465
Plasmid#158587PurposeEnzymes Bv-CYP76AD5, DODC, ARO4_K229LDepositorInsertsBv-CYP76AD5
DODC
ARO4_K229L
ExpressionYeastMutationK229LAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only