8,418 results
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Plasmid#128560PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert68Q-Httex1 (without proline-rich region) fused to ymsfGFP
TagsymsfGFPExpressionYeastPromoterGAL1Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-HphMx
Plasmid#122919PurposeLYS2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-NatMx
Plasmid#122921PurposeLYS2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0654
Plasmid#129015PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0623
Plasmid#128998PurposepRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0617
Plasmid#128993PurposeloxP-KIURA3-loxP preassembled entry vectorDepositorInsertKanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0624
Plasmid#128999PurposepZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
ExpressionYeastAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0621
Plasmid#128996PurposepRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1DepositorInsertConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
ExpressionYeastAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1671
Plasmid#112045PurposeCEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URADepositorInsertORM1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV13
Plasmid#128864PurposePosition 1 module- CEN (Centromeric)DepositorInsertCEN/ARS
UseSynthetic BiologyExpressionYeastAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA053
Plasmid#128515PurposeExpresses SFA8 in PichiaDepositorInsertSFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
ExpressionYeastPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV12
Plasmid#128893PurposePosition 6 module- NatMXDepositorInsertNatMX
UseSynthetic BiologyExpressionYeastAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only