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Plasmid#106143PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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pWHI2-NAT
Plasmid#80588PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterNative WHI2 promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH743-CEN-RLuc/slowstaCFLuc
Plasmid#38223DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH742-CEN-RLuc/slowminCFLuc
Plasmid#38222DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJT3
Plasmid#98304PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-81nmt1
Plasmid#105164PurposeYeast gene targetingDepositorInserthphMX6-81nmt1
UseTags81nmt1ExpressionBacterial and YeastMutationPromoter81nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-41nmt1
Plasmid#105163PurposeYeast gene targetingDepositorInserthphMX6-41nmt1
UseTags41nmt1ExpressionBacterial and YeastMutationPromoter41nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-ccdB-EYFP
Plasmid#14207DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pXP711
Plasmid#46053DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP712
Plasmid#46054DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorInsertMkk2DS NLS mCherry (MKK2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAG416GPD-Cerulean-YPT52
Plasmid#18843DepositorInsertYPT52 (YPT52 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
sup35-C653R
Plasmid#1198DepositorInsertSup35p (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationchanged cys 653 to argPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUDP010
Plasmid#101166PurposeE. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP4xV5_ccdB
Plasmid#79016PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB761
Plasmid#60156PurposeFirefly luciferase (Promega) driven by ADE17 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterADE17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCfB2991
Plasmid#63643PurposepCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only