8,388 results
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p414ADH_Hsp82_noTER
Plasmid#61715Purposeyeast plasmid with Hsp82DepositorAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail
Plasmid#60396PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB754
Plasmid#60157PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLYS9prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Neo 1-F GW
Plasmid#58545PurposeCEN vector with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS24-Pleum
Plasmid#60327PurposeContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB691
Plasmid#60135PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertRdLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
p416 3-F GW
Plasmid#58552PurposepRS416 carrying Gateway cassette for three insertsDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
TagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
p42Nat 3-F GW
Plasmid#58557PurposeMulticopy with clonNat resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Neo 3-F GW
Plasmid#58554PurposeCEN vector with G418 resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Nat 1-F GW
Plasmid#58546PurposeCEN vector with clonNat resistance marker for single Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain EE mutation
Plasmid#58833PurposeExpresses GFP-Tagged and mutated CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastMutationchanged Arginine 43 to GlutamatePromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only